摘要
目的 研究Survivin与Caspase-3基因在小儿肝母细胞瘤(HB)中的表达及其临床病理价值。方法 回顾性选择2011年6月至2013年9月在河北北方学院附属第一医院确诊的HB患儿42例,取HB样本作为实验样本、瘤旁样本作为对照。测定Survivin与Caspase-3基因的阳性表达率以及与HB患儿临床病理特征的关系;随访HB患儿5年总体生存率(OS)及无病生存率(EFS),分析不同Survivin、Caspase-3表达的HB患儿生存率的差异。结果 HB组织中Survivin的阳性表达率显著高于瘤旁组织,Caspase-3的阳性表达率显著低于瘤旁组织,差异有统计学意义(P <0. 05);HB组织中Survivin、Caspase-3的阳性表达率与患儿的年龄、性别、组织学分型无关,差异无统计学意义(P>0. 05),与患儿的肿瘤直径、临床分级、淋巴转移有关,差异有统计学意义(P <0. 05);Survivin阳性表达HB患儿的5年OS及EFS均显著低于Survivin阴性表达的HB患儿,Caspase-3阳性表达HB患儿的5年OS及EFS均显著高于Caspase-3阴性表达的HB患儿,差异有统计学意义(P <0. 05)。结论 Survivin基因的高表达与Caspase-3基因的低表达参与HB的发生且与HB的预后有关。
Objective To study the expression of Survivin and Caspase-3 gene in hepatoblastoma(HB)of children and its clinicopathological value.Methods HB samples from children with HB diagnosed in our hospital from June 2011 to September 2013 were selected as experimental samples and paraneoplastic samples were selected as control.The positive expression rates of Survivin and Caspase-3 genes were determined,the 5-year overall survival rate(OS)and event-free survival(EFS)were followed up.Results The positive expression rate of Survivin in HB tissues was significantly higher than that in paraneoplastic tissues,and the positive expression rate of Caspase-3 was significantly lower than that in paraneoplastic tissues,the difference was statistically significant(P<0.05).The positive expression rates of Survivin and Caspase-3 in HB tissues had no correlation with age,sex and histological type(P>0.05),which were correlated with tumor diameter,clinical grade and lymphatic metastasis(P<0.05).Five 5 years OS and EFS of HB patients with Survivin positive expression were significantly lower than those of HB patients with Survivin negative expression,and 5 years OS and EFS of HB patients with Caspase-3 positive expression were significantly higher than those of HB patients with Caspase-3 negative expression,the difference was statistically significant(P<0.05).Conclusion The high expression of Survivin gene and the low expression of Caspase-3 gene are involved in the occurrence of HB and the prognosis of HB.
作者
孙海珍
袁二伟
岳丽娟
张洪素
SUN Hai-zhen;YUAN Er-wei;YUE Li-juan(Department of Pediatric Internal Medicine,The First Affiliated Hospital of Hebei North University,Zhangjiakou Hebei 075000,China)
出处
《临床和实验医学杂志》
2020年第8期860-863,共4页
Journal of Clinical and Experimental Medicine
基金
河北省卫生和计划生育委员会重点课题(编号:20180852)。
