摘要
马铃薯病毒病是导致马铃薯退化的主要原因之一,寻找能够有效降低病毒病对马铃薯生产的影响一直是马铃薯研究和生产者的追求。通过研究马铃薯中早熟品种‘LK99’和晚熟品种‘陇薯3号’对马铃薯Y病毒(PVY)和马铃薯卷叶病毒(PLRV)的生理反应,进一步明确和比较了中早熟品种‘LK99’和晚熟品种‘陇薯3号’在PVY或PLRV胁迫下的一些生理变化。以未感病和分别感染了PVY、PLRV的马铃薯中早熟品种‘LK99’和晚熟品种‘陇薯3号’为研究对象,在马铃薯苗期、块茎形成期、块茎膨大期、淀粉积累期采用紫外分光光度计测定了所取叶片中超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性、丙二醛(MDA)含量、叶绿素含量,并进行了分析比较;同时,在块茎膨大期测定株高、茎粗、地上鲜重,成熟后测产。试验结果表明,病毒侵染马铃薯植株后,植株生长受抑制,块茎产量降低、有关保护酶(SOD、POD、CAT)活性增强、丙二醛含量升高、总叶绿素含量减少。植株感染了PVY或PLRV后块茎产量、有关保护酶(SOD、POD、CAT)活性、丙二醛含量、总叶绿素含量的相对变化量表明‘陇薯3号’的受损程度较‘LK99’严重。以块茎产量作为评价标准,则PVY对马铃薯的危害程度较PLRV更为严重。马铃薯植株对PLRV的应激性反应比对PVY强烈,表现为酶活相对增强幅度大、丙二醛含量相对增量高、总叶绿素含量相对减量高。
Potato viral disease is one of the main reasons leading to the deterioration of potato and the search for effective ways to reduce the effects of viral diseases was a pursuit of both potato researchers and producers. The physiological responses of the early maturing variety ’LK99’ and the late maturing variety ’Longshu 3’ to potato virus Y(PVY) and potato leaf roll virus(PLRV) were studied, and the physiological changes of the early maturing variety ’LK99’and the late maturing variety ’Longshu 3’ under the stresses of PVY or PLRV were further clarified and compared using normal, PVY and PLRV infected potatoes from ’LK99’ and ’Longshu 3’ respectively as the test materials. Superoxide dismutase(SOD), peroxidase(POD), catalase(CAT) activity, malondialdehyde(MDA) content and chlorophyll content were determined and analyzed by ultraviolet spectrophotometer at the seedling, tuber formation, tuber bulking and starch accumulation stages. At the same time, plant height, stem diameter and fresh weight above ground were measured at the tuber bulking stage, and potato yield were measured at mature stage. After potato plants were infected with viruses, the growth was inhibited, tuber yield was reduced, activity of relevant protective enzymes(SOD, POD and CAT) were increased, MDA content was increased and total chlorophyll content was reduced. After potato plants were infected with PVY or PLRV, the relative changes of tuber yield, protective enzyme activity(SOD, POD and CAT), MDA content and total chlorophyll content showed that the damage to ’Longshu 3’ was more serious than that of ’LK99’. When tuber yield was used as the evaluation standard, the harm caused by PVY to potato was more serious than that of PLRV.The stress response of potato plants to PLRV was stronger than that of PVY, which were manifested as a large increase in protective enzyme activity(SOD, POD and CAT) and MDA content, and a large decrease in the total chlorophyll content.
作者
张武
吴雁斌
高彦萍
梁宏杰
吕和平
ZHANG Wu;WU Yanbin;GAO Yanping;LIANG Hongjie;LU Heping(Potato Research Institute,Gansu Academy of Agricultural Sciences,Lanzhou,Gansu 730070,China;Gansu Engineering Center for Virus Detection and Safety Evaluation of Potato Virus-free Seed(Seedling),Lanzhou,Gansu 730070,China)
出处
《中国马铃薯》
2020年第2期103-113,共11页
Chinese Potato Journal
基金
甘肃省农业科学院农业科技创新专项项目(2019GAAS04)
国家重点研发计划项目(2017YFD0201602-4,2018YFD0200805)
兰州市人才创新创业项目(2016-RC-65)。
关键词
马铃薯病毒
保护酶活性
叶绿素
丙二醛
potato virus
protective enzyme activity
chlorophyll
malondialdehyde
