摘要
目的分析白介素17(IL-17)、白介素23(IL-23)及白介素35(IL-35)在稽留流产患者血清中的表达及意义。方法选取2016年7月~2019年2月在医院行清宫术的稽留流产妇女96例为研究组、同期正常妊娠妇女80例为对照组,采用酶联免疫吸附试验(ELISA)测定两组血清IL-17、IL-23及IL-35水平,比较稽留流产妇女中流产原因明确与流产原因未明者、不同孕周患者、不同预后患者入院血清IL-17、IL-23及IL-35水平,分析其对预后的预测价值。结果研究组血清IL-17、IL-23水平高于对照组,而血清IL-35水平较对照组低(P <0.05);流产原因明确者血清IL-17、IL-23水平高于流产原因未明者,流产原因明确者血清IL-35水平低于流产原因未明者(P <0.05);稽留流产者中,孕周>14 w者血清IL-17、IL-23水平高于孕周≤10 w和孕周11~14 w者(P <0.05),孕周>14 w者血清IL-35水平低于孕周≤10 w和孕周11~14 w者(P <0.05),孕周≤10 w和孕周11~14 w者上述指标比较差异也有统计学意义(P <0.05);流产术后6个月,宫腔粘连者血清IL-17、IL-23水平高于宫腔无粘连者,而IL-35水平低于宫腔无粘连者(P <0.05);血清IL-17、IL-23及IL-35水平预测稽留流产者术后宫腔粘连的ROC曲线下面积分别为0.775、0.696、0.566,三者联合预测价值最高,曲线下面积达0.863。结论稽留流产患者血清IL-17、IL-23高表达,而IL-35低表达,且流产原因明确、孕周>14 w者上述指标变化最大,IL-17、IL-23及IL-35水平也能预测患者预后,应加以监测。
Objective To explore the expression and significance of interleukin-17(IL-17),interleukin-23(IL-23)and interleukin-35(IL-35)in serum of patients with missed abortion.Methods A total of 96 women with missed abortion who underwent uterine curettage in our hospital were included as the study group,and 80 normal pregnant women were included as the control group.The levels of serum IL-17,IL-23 and IL35 of the two groups were determined using the enzyme-linked immunosorbent assay(ELISA).The levels of serum IL-17,IL-23 and IL-35 at the time of admission were compared between women with missed abortion with clear cause of abortion and those with unknown cause of abortion,among women with different gestational ages and among women with different prognosis.The value of levels of serum IL-17,IL-23 and IL-35 at the time of admission in predicting the prognosis was analyzed.Results The levels of serum IL-17 and IL-23 in the study group were higher than that in the control group,and the level of serum IL-35 was lower(P<0.05).The levels of serum IL-17 and IL-23 in women with clear cause of abortion were higher than that in the women with unknown cause of abortion,and the level of serum IL-35 was lower(P<0.05).Among women with missed abortion,the levels of serum IL-17 and IL-23 in women with a gestational age greater than 14 weeks were higher than that in those with a gestational age less than or equal to 10 weeks and those with a gestational age between 11 weeks and 14 weeks(P<0.05),and the level of serum IL-35 was lower(P<0.05).The difference of the above indicators between the women with a gestational age less than or equal to 10 weeks and women with a gestational age between 11 weeks and 14 weeks was also statistically significant(P<0.05).At six months after abortion surgery,the levels of serum IL-17 and IL-23 in women with intrauterine adhesion were higher than that in those without intrauterine adhesion,and the level of serum IL-35 was lower(P<0.05).The areas under ROC curve(AUC)of levels of serum IL-17,IL-23 and IL-35 for predicting postoperative intrauterine adhesion in patients with missed abortion were 0.775,0.696 and 0.566,respectively.The combination of these three biomarkers produced the highest predicted value,with the AUC up to 0.863.Conclusion IL-17 and IL-23 are highly expressed in the serum of patients with missed abortion,while IL-35 is lowly expressed.Women with clear cause of abortion and a gestational age of greater than 14 weeks show the greatest change in the above indicators.The level of serum IL-17,IL-23 and IL-35 can also predict prognosis of patients,which should be monitored.
作者
米思蓉
张宁梅
罗苗苗
艾庆燕
赵豫凤
Mi Sirong;Zhang Ningmei;Luo Miaomiao;Ai Qingyan;Zhao Yufeng(Department of Laboratory Mediceine,Yan'an University Affiliated Hospital,Yan'an,Shaanxi,716000,China;School of Medicine,Yan'an University,Yan an,Shaanxi,716000,China;Obstetrics Ward 1,Yan'an University Afiliated Hospital,Yan'an,Shaanxi,716000,China)
出处
《西南国防医药》
CAS
2020年第5期414-417,共4页
Medical Journal of National Defending Forces in Southwest China
基金
国家科技创新项目(201910719024)
延安市科技项目(2013-KW03).
