摘要
目的探究白藜芦醇(RSV)通过即刻早期基因(c-fos)通路对急性心肌梗死(AMI)大鼠心肌损伤标志物含量的影响。方法选取48只SD大鼠随机分为Sham组、AMI组和AMI+RSV组,每组各16只。通过结扎建立AMI模型,AMI+RSV组使用RSV灌胃干预(10 mg·kg/d)。检测各组大鼠心功能和心肌损伤标志物肌酸激酶MB(CK-MB)和乳酸脱氢酶(LDH)。HE染色和TUNEL染色检测组织损伤和心肌细胞凋亡。Western blot检测细胞外调节蛋白激酶(ERK)、磷酸化的ERK(p-ERK)和c-fos水平。结果AMI组大鼠左室射血分数(LVEF)及左室内压力最大变化率(±dp/dtmax)水平显著低于Sham组(P<0.05),AMI+RSV组LVEF、±dp/dtmax水平显著高于AMI组(P<0.05)。AMI组CK-MB和LDH水平显著高于Sham组(P<0.05),AMI+RSV组CK-MB和LDH水平显著低于AMI组(P<0.05)。AMI组凋亡率(38.75%±4.36%)显著高于Sham组(4.54%±0.58%,P<0.05),AMI+RSV组心肌细胞凋亡率(19.67%±4.18%)显著低于AMI组(P<0.05)。AMI组p-ERK/ERK和c-fos蛋白水平显著高于Sham组(P<0.05),AMI+RSV组p-ERK/ERK和c-fos蛋白水平显著低于AMI组(P<0.05)。结论RSV通过调节c-fos通路抑制AMI模型大鼠的心肌细胞凋亡,缓解心肌细胞损伤并发挥保护AMI后心功能的作用。
Objective To study the influence of resveratrol(RSV)on content of myocardial damage biomarker through pathway of immediate early gene(c-fos)in rats with acute myocardial infarction(AMI).Methods SD rats(n=48)were divided randomly into Sham group,AMI group and AMI+RSV group(each n=16).AMI model was established through ligation,and AMI+RSV group was intragastrically intervened with RSV(10 mg·kg/d).The biomarkers of heart function and myocardial damage,including creatine kinase-MB isoenzyme(CK-MB)and lactic dehydrogenase(LDH),were detected in all groups.The myocardial tissue damage and cardiomyocyte apoptosis were detected after HE staining and TUNEL staining.The levels of extracellular regulated protein kinases(ERK),phosphorylated ERK(p-ERK)and c-fos were detected by using Western blotting assay.Results The levels of left ventricular ejection fraction(LVEF)and the maximum rate of change of left ventricular pressure(±dp/dtmax)were significantly lower in AMI group than those in Sham group(P<0.05),and were significantly higher in AMI+RSV group than those in AMI group(P<0.05).The levels of CK-MB and LDH were significantly higher in AMI group than those in Sham group(P<0.05),and were significantly lower in AMI+RSV group than those in AMI group(P<0.05).The apoptosis rate was significantly higher in AMI group(38.75%±4.36%)than that in Sham group(4.54%±0.58%,P<0.05)and was significantly lower in AMI+RSV group(19.67%±4.18%)than that in AMI group(P<0.05).The levels of p-ERK/ERK and c-fos were significantly higher in AMI group than those in Sham group(P<0.05),and were significantly lower in AMI+RSV group than those in AMI group(P<0.05).Conclusion RSV can inhibit myocardial apoptosis,relieve myocardial damage and protect heart function after AMI through regulating c-fos pathway in rat AMI model.
作者
李成军
董梅
任法新
Li Chengjun;Dong Mei;Ren Faxin(Medical School,Qingdao University,Qingdao 266071,China)
出处
《中国循证心血管医学杂志》
2020年第4期436-438,442,共4页
Chinese Journal of Evidence-Based Cardiovascular Medicine
基金
山东省自然科学基金联合专项(ZR2018HL004)。
关键词
急性心肌梗死
凋亡
心肌损伤
即刻早期基因
大鼠
Acute myocardial infarction
Apoptosis
Myocardial damage
Immediate early gene
Rats
