摘要
目的探讨真核起始因子3b(eIF3b)在乳腺癌中的表达及与患者临床特征的关系。方法取45例乳腺癌患者的乳腺癌组织和相应的癌旁组织,采用实时定量聚合酶链反应(RT-PCR)检测乳腺癌组织和相应的癌旁组织eIF3b mRNA的相对表达量,采用免疫组化二步法检测乳腺癌组织和相应的癌旁组织e IF3b蛋白的阳性表达率,分析e IF3b mRNA的相对表达量和eIF3b蛋白的阳性表达率与乳腺癌患者临床特征的关系。结果乳腺癌组织中eIF3b m RNA的相对表达量和eIF3b蛋白的阳性表达率,均明显高于癌旁组织,差异均有统计学意义(P﹤0.01)。不同年龄、病理类型、肿瘤直径、组织学分级乳腺癌患者乳腺癌组织中eIF3b mRNA相对表达量和e IF3b蛋白阳性表达率的比较,差异均无统计学意义(P﹥0.05);不同腋窝淋巴结转移情况、TNM分期乳腺癌患者乳腺癌组织中eIF3b mRNA相对表达量和eIF3b蛋白阳性表达率的比较,差异均有统计学意义(P﹤0.05)。结论 eIF3b表达增高不仅可能参与了乳腺癌发生过程,也可能在乳腺癌的增殖、转移和侵袭过程中发挥促进作用。
Objective To investigate the expression of eukaryotic initiation factor 3b(eIF3b) in breast cancer and to determine the correlation with clinicopathological characteristics.Method The breast cancer tissues and adj acent normal tissues from 45 cases with breast cancer were collected,the relative expression of eIF3b mRNA was detected with reverse transcription-polymerase chain reaction(RT-PCR) and protein expression was determined using a two-step immunohistochemistry assay.The correlation of relative expression of eIF3b mRNA and positive expression rate of eIF3b protein with the clinicopathological characteristics of breast cancer patients was studied.Result The expression of eIF3b mRNA and protein in breast cancer tissues were significantly higher than those in adjacent tissues(P<0.01),while were independent of age,pathological type,tumor diameter,histological classification among breast cancer patients(P>0.05);though axillary lymph node metastasis and TNM stage were implicated with eIF3b mRNA and protein expression in breast cancer patients(P<0.05).Conclusion Increased eIF3b might not only be involved in the occurrence of breast cancer,but also participate in the proliferation,metastasis and invasion of breast cancer.
作者
李延辉
方茅
谢敏如
夏明汗
LI Yanhui;FANG Mao;XIE Minru;XIA Minghan(Department of Pathology,Shijie Hospital,Dongguan 523290,Guangdong,China;Department of Teaching and Research Office of Pathology,Guangzhou Medical College,Guangzhou 510000,Guangdong,China;Department of Pathology,the First Affiliated Hospital of Ji’nan University,Guangzhou 510000,Guangdong,China)
出处
《癌症进展》
2020年第8期788-790,共3页
Oncology Progress
基金
2019年东莞市社会科技发展(重点)项目(201950715011207)。
关键词
乳腺癌
聚合酶链反应
免疫组织化学
真核起始因子3b
breast carcinoma
polymerase chain reaction
immunohistochemistry
eukaryotic initiation factor 3b
