摘要
目的研究鱼油和红花籽油对骨质疏松症模型小鼠脂肪酸合成的影响及作用机制。方法对8周龄雌性健康C57BL/6J小鼠进行双侧去卵巢手术,建立骨质疏松症模型,术后3 d随机分为假手术组(生理盐水)、模型组(生理盐水)和FO组(40 mg·kg^-1·bw^-1)、SO组(40 mg·kg^-1·bw^-1)。连续灌胃14周后取材,检测各组小鼠骨密度、骨生物力学、炎症因子等相关指标,qRT-PCR检测肝脏中脂肪酸合成因子mRNA的表达水平。结果鱼油显著增加骨强度,降低模型小鼠体脂比、血清总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)等脂代谢表观指标,降低n-6多不饱和脂肪酸(PUFAs)代谢中间产物——前列腺素E2(prostaglandin,PGE2)、前列腺素E受体4(prostaglandin E receptor,EP4)以及下游炎症基因—核转录因子κB(nuclear factorκB,NF-κB)、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)的表达水平。由NF-κB介导的脂肪酸合成关键基因mRNA表达水平也显著下降:包括过氧化酶增殖因子活化受体γ(peroxisome proliferator-activated receptor gamma,PPARγ)、固醇调节元件结合蛋白-1c(sterol regulatory element binding protein-1c,SREBP-1c)、脂肪酸合成酶(fatty acid synthase,FAS)、乙酰辅酶A羧化酶(acetyl-CoA carboxylase,ACC)。红花籽油降低骨强度,显著提高模型小鼠体脂比、TC、PGE2含量以及脂肪酸合成调控基因SREBP-1c的mRNA表达水平。结论提示通过介导PPARγ/NF-κB炎症通路,鱼油显著改善模型小鼠的脂肪酸合成,而红花籽油介导NF-κB炎症通路使模型小鼠的脂肪酸合成发生紊乱。
Objective In this study,the mechanism of fish oil(FO)and safflower seed(SO)oil on fatty acid synthesis in osteoporosis mice induced by ovariectomized were investigated in vivo.Methods Female C57 BL/6 J mice were ovariectomized to establish the osteoporotic model.After 3 days,they were randomly assigned into four groups,including sham-operated group(normal saline),model group(normal saline),FO group(40 mg·kg^-1 bw^-1)and SO group(40 mg·kg^-1 bw^-1).After 14 weeks treatment,the bone mineral density(BMD),tibia biochemical properties,inflammation factors were measured,and the mRNA expressions of fatty acid synthase factor in liver tissue of mice were assayed with qRT-PCR.Results FO significantly prevents bone loss,up-regulates the body fat percentage,total cholesterol(TC),triglyceride(TG),and reduces n-6 PUFAs metabolic intermediates-prostaglandin E2(PGE2),Prostaglandin E receptor 4(EP4),as well as the low expression levels of downstream inflammatory genes-nuclear factorκB(NF-κB),tumor necrosis factor-α(TNF-α).The mRNA expression level of key genes involved in fatty acid synthesis mediated by NF-κB also decreased significantly,including peroxisome proliferator-activated receptor gamma(PPARγ),serol regulatory element binding protein-1 c(SREBP-1c),fatty acid synthase(FAS),Acetyl-CoA carboxylase(ACC).SO significantly down-regulated bone mass,increased the body fat percentage,TC,PGE2 levels and the mRNA expression levels of SREBP-1c in fatty acid synthesis regulatory gene.Conclusion Fish oil significantly improved lipid accumulation while safflower seed oil caused fatty acid synthesis disorder in ovariectomized mice,which mediated by the PPARγand NF-κB inflammatory pathway.
作者
符萌
张雷
李雪静
韩立华
王静凤
FU Meng;ZHANG Lei;LI Xue-jing;HAN Li-hua;WANG Jing-feng(College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China)
出处
《中国海洋药物》
CAS
CSCD
2020年第2期50-58,共9页
Chinese Journal of Marine Drugs
基金
国家重点研发计划项目(2018YFC0311203)资助。
