摘要
目的分析病理组织过碘酸雪夫氏(periodic acid Schiff,PAS)染色的影响因素,以期提高其染色效果。方法选取已知含有PAS阳性物质的胃粘膜组织,分别制作成蜡块,通过对比试验,比较高碘酸水溶液不同染色时长、雪夫氏(Schiff)试剂不同配制方法、Schiff试剂不同保存时间对PAS染色质量的影响。结果胃粘膜组织在使用高碘酸水溶液染色11~15min优于其他时长,Schiff试剂热配法优于冷配法,试剂保存2个月内的染色效果优于试剂保存大于2个月者。结论胃粘膜组织使用高碘酸水溶液染色11~15min、热配法配制的Schiff试剂、保存在2个月内的Schiff试剂进行染色效果最佳。
Objective To analyze the influence factors of pathological tissue Periodic Acid Schiff(PAS) staining and in order to improve its staining quality. Methods The gastric mucosa tissues with known PAS-positive substances were selected and made into paraffin blocks. The contrast tests were used to compare effect of different staining duration of aqueous periodate solution, different preparation methods of Schiff reagent and different storage time of Schiff reagent on the PAS staining quality. Results The 11~15 min staining duration of aqueous periodate solution for gastric mucosa tissue was optimum. The hot-mixing method of Schiff reagent was better than the cold-mixing method. The staining quality of the reagent with storage time within 2 months was better than that of the reagent with storage time more than 2 months. Conclusion The staining quality was optimum when gastric mucosa tissue was stained with aqueous periodate for 11~15 min, with Schiff reagent prepared by hot-mixing method and stored within 2 months.
作者
林倩
方庆全
Lin Qian;Fang Qingquan(Department of Pathology,The First Affiliated Hospital of Xiamen University,Xiamen 361003,China)
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2020年第1期77-80,共4页
Chinese Journal of Histochemistry and Cytochemistry
关键词
PAS
染色
影响因素
试剂
配制方法
PAS staining
influence factors
reagent
preparation methods
