摘要
目的探讨MAST3对胃癌细胞SGC-7901的增殖及迁移作用。方法使用Western blot及qRT-PCR法检测MAST3在胃癌组织中的表达情况,进一步将MAST3-RNAi和pEx-3-MAST3转染到SGC-7901细胞中,分别使用qRT-PCR、Western blot检测CyclinD1、C-myc及MMP-9、MMP-3的表达,观察MAST3对SGC-7901的增殖及迁移作用;通过流式细胞仪检测细胞周期。结果 Western blot结果显示,MAST3在胃癌组织中高于癌旁组织(P<0.01),且在TGF-β1刺激后表达也随之增加。使用MAST3-RNAi抑制MAST3表达后,CyclinD1和C-myc蛋白表达明显低于对照组(P<0.01),且S期和G2/M期细胞明显降低,同时MMP9和MMP3蛋白表达也明显低于对照组;使用pEx-3-MAST3过表达MAST3后,CyclinD1和C-myc蛋白表达明显高于对照组(P<0.01),且S期和G2/M期细胞升高,MMP-9和MMP-3蛋白表达也明显高于对照组。结论 MAST3能够调控胃癌细胞SGC-7901细胞的增殖及迁移能力。
Objective To investigate the effect of MAST3 on proliferation and migration in SGC-7901 cells.Methods Western blot and qRT-PCR were used to detect MAST3 expression in gastric cancer tissues. MAST3-RNAi and p Ex-3-MAST3 were further transfected into SGC-7901 cells,and the expressions of CyclinD1,C-myc,MMP9 and MMP3 were detected by qRT-PCR and Western blot,respectively,and the effect of MAST3 on the proliferation and migration of SGC-7901 cells was observed. Cell cycle was detected by flow cytometry. Results Western blot results showed that MAST3 was significantly higher in gastric cancer tissues than that in adjacent tissues( P < 0. 01),and the expression increased significantly after TGF-β1 stimulation. After MAST3-RNAi inhibited the expression of MAST3,the expression of CyclinD1 and C-myc protein was significantly lower than that of the control group( P < 0. 01),and the cells in S phase and G2/M phase significantly decreased,while the expression of MMP-9 and MMP-3 protein was also significantly lower than that of the control. The expression of CyclinD1 and Cmyc protein was significantly higher than that of the control group after p Ex-3-MAST3 over-expression of MAST3( P< 0. 01),and the cells in S phase and G2/M phase significantly increased,MMP-9 and MMP-3 protein expression was also significantly higher than of the control group. Conclusion MAST3 can regulate the proliferation and migration of gastric cancer SGC-7901 cells.
作者
王磊
陈鹤
张敏
王中新
Wang Lei;Chen He;Zhang Min(Dept of Clinaai Laborators,The First Affiliated Hospital of Anhui MePical Unwersnh,Hefei 230022)
出处
《安徽医科大学学报》
CAS
北大核心
2020年第4期523-528,共6页
Acta Universitatis Medicinalis Anhui
基金
安徽高校自然科学研究项目(编号:KJ2017A208)。
