摘要
目的比较牙周健康者和牙周炎患者牙龈组织中B细胞、浆细胞及其相关细胞因子表达的差异。方法采集牙周健康者(牙周健康组,n=12)、牙周炎患者(牙周炎组,n=15)的牙龈组织,通过苏木精-伊红(HE)染色进行组织病理学检测,免疫组织化学染色(CD19、CD38和CD138)检测B细胞和浆细胞的表达,酶联免疫吸附测定法检测B细胞活化因子(BAFF)和可溶性核因子κB受体活化因子配体(sRANKL)的表达。结果牙周炎组牙龈结缔组织内大量炎症细胞浸润;CD19(+)、CD38(+)和CD138(+)细胞数量高于牙周健康组(P<0.000 1);BAFF和sRANKL的表达均高于牙周健康组(P值分别<0.01和<0.001)。结论牙周炎患者的B细胞、浆细胞及其相关细胞因子BAFF和sRANKL的表达均增高,提示B细胞和浆细胞可能参与了牙周炎的进程。
Objective This study aimed to compare the differences of B cells,plasma cells,and related cytokines expression in gingival tissues between periodontitis and periodontal healthy subjects.Methods Gingival tissues were collected from periodontal healthy subjects(periodontal healthy group,n=12)and periodontitis patients(periodontitis group,n=15).Hematoxylin-eosin(HE)staining was used for histopathological examination.Immunohistochemical staining(CD19,CD38,and CD138)was applied to detect the expression of B cells and plasma cells.B cell-activating factor(BAFF)and soluble receptor activator of nuclear factor-κB ligand(sRANKL)were detected by enzyme-linked immunosorbent assay.Results Extensive inflammatory cell infiltration was found in the gingival tissues of the periodontitis group.The number of CD19(+),CD38(+),and CD138(+)cells of the periodontitis group was significantly higher than that of the periodontal healthy group(P<0.0001).BAFF and sRANKL levels of the periodontitis group were higher than those of the periodontal healthy group(P<0.01,P<0.001,respectively).Conclusion The expression of B cells,plasma cells,and their related BAFF and sRANKL cytokines were significantly higher in periodontitis patients than those in the periodontal healthy subjects,suggesting that B cells and plasma cells may be involved in the development of periodontitis.
作者
欧阳玉玲
陈盛
陈斌
Ouyang Yuling;Chen Sheng;Chen Bin(Dept.of Periodontology,Shanghai Jing-an Dental Clinic,Shanghai 200040,China;Dept.of Pathology,Nanjing Stomatological Hospital,Medical School of Nanjing University,Nanjing 210008,China;Dept.of Periodontology,Nanjing Stomatological Hospital,Medical School of Nanjing University,Nanjing 210008,China)
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2020年第3期250-255,共6页
West China Journal of Stomatology
基金
南京市医学科技发展资金(QRX17176)。
