摘要
目的探讨妊娠期糖尿病(GDM)患者血清miR-149水平与胰岛素抵抗的关系。方法选取2017年1月~2018年8月陕西省人民医院收治的63例妊娠期糖尿病患者设为观察组,选择本院同期产检健康妊娠产妇63例设为对照组,用自动糖化血红蛋白检测仪测定糖化血红蛋白(HbA1c),用生化分析仪检测受试者餐前空腹血糖(FPG),用免疫测定分析仪检测血清空腹胰岛素(FINS),计算胰岛素抵抗指数(HOMA-IR)=FPG×FINS/22.5。采用实时荧光定量PCR(qRT-PCR)法检测所有研究对象血清miR-149表达水平。用Pearson分析miR-149与血糖、胰岛素等指标的相关性。对影响胰岛素抵抗的相关因素进行Logistic回归分析。结果与对照组相比,观察组孕妇HOMA-IR、FPG、FINS、HbA1C水平、miR-149水平较高(P<0.05);Pearson法分析显示观察组孕妇血清miR-149水平与FPG、FINS、HOMA-IR呈正相关(P<0.05),与HbA1C无相关性(P>0.05);多因素Logistic回归分析显示血清miR-149水平升高是影响胰岛素抵抗的危险因素。结论妊娠期糖尿病患者血清miR-149水平上调,可能与妊娠期糖尿病孕妇胰岛素抵抗有关。
Objective To investigate the relationship between level of serum miR-149 and insulin resistance in patients with gestational diabetes mellitus(GDM).Methods 63 gestational diabetes mellitus treated in our hospital from January 2017 to August 2018 were selected as observation group.During the same period,63 healthy pregnant women Underwent maternity examination in our hospital were selected as control group.The levels of glycosylated hemoglobin(HbA1c)were measured by automatic glycosylated hemoglobin analyzer.The fasting blood glucose(FPG)was measured by biochemical analyzer.The fasting insulin(FINS)was measured by immunoassay analyzer,and insulin resistance index(HOMA-IR)=FPG*FINS/22.5 was calculated.Real-time fluorescence quantitative PCR(qRT-PCR)was used to detect the level of serum miR-149 expression in all subjects.Pearson was used to analyze the correlations between miR-149 and blood sugar,insulin and other indicators.Logistic regression analysis was used to analyze the related factors affecting insulin resistance.Results Compared with the control group,the levels of HOMA-IR,FPG,FINS,HbA1C and miR-149 were higher than that in the observation group(P<0.05).Pearson analysis showed that the level of serum miR-149 was positively correlated with FPG,FINS and HOMA-IR(P<0.05),but not with HbA1C(P>0.05).The multivariate logistic regression analysis showed that the high level of serum miR-149 was a risk factor for insulin resistance.Conclusion The level of serum miR-149 in patients with gestational diabetes mellitus is up-regulated,which may be related to insulin resistance in pregnant women with gestational diabetes mellitus.
作者
董春萍
吴贵福
张雅
高珊
DONG Chunping;WU Guifu;ZHANG Ya;GAO Shan(Department of Endocrinology, Shanxi people′s Hospital, Xi′an 710068, China;department of Obstetrics, Shanxi people′s Hospital, Xi′an 710068, China)
出处
《西部医学》
2020年第5期700-703,共4页
Medical Journal of West China
