摘要
采用三重四级杆串联质谱法(UPLC-MS/MS)分析淫羊藿大花类群总状花序品种与圆锥状花序品种间主要黄酮成分含量差异。质谱采用电喷雾离子源正离子模式(ESI^+)采集,通过多反应监测(MRM)同时对淫羊藿药材中的14种有效成分进行定量分析。含测结果显示圆锥状花序品种黄酮成分含量要明显高于总状花序,而朝鲜淫羊藿成分含量及变化趋势则异于其它花序品种;聚类结果显示,总状花序品种与圆锥状花序品种区分明显,朝鲜淫羊藿在PCA及PLS-DA的得分图中被单独归为一类,与含量分析结果一致,这与朝鲜淫羊藿归于淫羊藿属sect.Macroceras组,而其它中国种类属于sect.Diphyllon组有关。该检测方法灵敏、准确、快速,适用于淫羊藿的定量分析,该实验结果为淫羊藿质量控制、品种选育等提供科学支撑。
The difference of flavonoid content between raceme and panicle varieties of large-flowered taxa of Epimedium was analyzed by UPLC-MS/MS.Mass spectrometry was collected by electrospray ion source positive ion mode(ESI^+),and quantitative analysis of 14 active ingredients in Herba Epimedii was carried out by multiple reaction monitoring(MRM).The results showed that the content of flavonoids in the panicle was significantly higher than that in the raceme.The content and variation trend of E.koreanum were different from other inflorescence varieties.The results of clustering showed that the racemes and panicles were clearly distinguished,and the E.koreanum was classified as a category in PCA and PLS-DA,which was consistent with the results of content analysis.This was consistent with E.koreanum belonging to sect. Macroceras group,while other Chinese species belonged to sect.Diphyllon group.The method is sensitive,accurate and rapid,and suitable for quantitative analysis of Epimedium.The results provide scientific support for quality control and breed selection of Epimedium herb.
作者
秦伟瀚
危永胜
阳勇
李卿
刘翔
QIN Wei-han;WEI Yong-shen;YANG Yong;LI Qin;LIU Xiang(Chongqing Institute of Traditional Chinese Medicine,Chongqing 400065,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2020年第4期633-644,共12页
Natural Product Research and Development
基金
国家重大新药创制项目(2017ZX09101002-002-004)
重庆市科技局项目(cc-cstc-cA-19-2、cstc2019jxjl-jbky0017)
重庆市自然基金项目(cstc2019jcyj-msxmX0464、cstc2018jcyjAX0316)
重庆市卫生局项目(ZY201802117)。
