摘要
旨在探究大足黑山羊妊娠早期胎儿胎盘血管发育、分布和血管生成相关因子表达状况,并对血管生成相关因子表达之间和血管发育进行相关性分析。本研究随机选取15只8月龄、体格相近、身体健康的国家级畜禽遗传资源—大足黑山羊青年母羊,经同一种公羊自然交配后(以末次配种当天记为0 d),经剖腹产手术采集妊娠第20、25和30天的胎儿和胎儿胎盘以及经屠宰采集妊娠第45和60天的胎儿和胎儿胎盘作为研究对象,通过免疫组化法评估胎盘血管发育和分布情况,采用qRT-PCR技术检测主要血管生成相关基因的mRNA表达水平,分析血管生成相关基因之间以及与胎盘血管发育分布的相关性。结果显示,胎儿体重和体长随着妊娠的进行逐渐增加,且血管内皮细胞阳性率处于较高水平(>26%)。山羊胎儿胎盘毛细血管总面积/组织区域面积(CAD)逐渐升高;毛细血管总数/单位组织区域面积(CND)在妊娠25~30 d增加,30~60 d降低,毛细血管总面积/毛细血管根数(APC)呈相反趋势;毛细血管总周长/单位组织区域面积(CSD)无显著变化。妊娠早期胎儿胎盘VEGFA的表达量随着妊娠的进行逐渐升高,且与血管分布存在一定的相关性,其受体FLT1和KDR表达量呈先增高后降低的趋势,分别在30和45 d达到峰值。血管生成相关基因ANGPT1/2及其受体TEK和FGF2及其受体FGFR2的表达均在妊娠30 d时较高。综上表明,在妊娠30 d前,胎儿胎盘主要通过毛细血管形成分支促进血管面积的增加;而在30~60 d,主要通过单位血管的增粗促进血管面积的增加,并且血管生成相关基因的表达在妊娠30 d时较高,这可能和子叶开始形成有关。山羊妊娠早期胎儿胎盘中VEGFA可能通过其受体结合与其他血管生成因子共同作用调控胎儿胎盘血管形成。
The study aimed to investigate the development and distribution of blood vessels and the expression of angiogenesis-related factors in fetal placenta of Dazu Black goat during early pregnancy,and to analyze the correlation between the expression of angiogenesis-related factors and vascular development.Fifteen 8-month-old,healthy,young female Dazu Black goats were randomly selected,after natural mating with the same ram(recorded as 0 d on the last breeding day),fetuses and fetal placenta were collected by cesarean operation at 20,25,and 30 d of pregnancy,and fetuses and fetal placenta were collected after slaughter at 45 and 60 d of pregnancy.Placental vascular development and distribution were investigated by immunohistochemical technology,and the expression level of angiogenesis-related genes was detected by qRT-PCR technique,and the correlation between the expression of angiogenesis-related genes and the placental vascular distribution was analyzed.The results showed that fetal body weight and body length increased gradually with the progress of pregnancy,and the positive rate of vascular endothelial cells was at a high level(>26%).With the progress of pregnancy,goat fetal placenta vascularity area/tissue area(CAD)gradually increased;capillary number/tissue area(CND)increased during 25-30 d and decreased during 30-60 d of pregnancy;Average capillary area(APC)showed an opposite trend to CND;There was no significant change in capillary circumference/tissue area(CSD).The expression of VEGFA in fetal placenta was gradually increased with the progress of pregnancy and correlated with vascular distribution.The expression level of FLT 1 and KDR in fetal placenta first increased and then decreased,reaching the peak at 30 and 45 d of pregnancy,respectively.The expression of ANGPT 1/2 and its receptor TEK,FGF 2 and its receptor FGFR 2 were higher at 30 d of pregnancy,which could be related to angiogenesis.In conclusion,before 30 d of pregnancy,fetal placenta mainly formed branches through capillaries to promote the increase of vascular area;at 30-60 d of pregnancy,the increase of vascular area was mainly promoted by the thickening of blood vessels per unit,and the expression of angiogenesis-related genes was higher at 30 d of pregnancy,which might be related to cotyledon formation.VEGFA in goat fetal placenta during early pregnancy could act together with other angiogenesis-related factors to regulate the angiogenesis.
作者
程文强
王攀
罗南剑
徐恢仲
王炜
张蓉
成磊
赵威
赵永聚
CHENG Wenqiang;WANG Pan;LUO Nanjian;XU Huizhong;WANG Wei;ZHANG Rong;CHENG Lei;ZHAO Wei;ZHAO Yongju(Chongqing Engineering Research Center for Herbivores Resource Protection and Utilization,Chongqing Key Laboratory of Forage&Herbivore,College of Animal Science and Technology,Southwest University,Chongqing 400715,China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2020年第5期1019-1029,共11页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家重点研发计划课题(2018YFD0502000)
国家自然科学基金(31772564)
重庆市技术创新与应用发展专项(cstc2019jscx-lyjsAX0018)。
关键词
大足黑山羊
妊娠早期
胎儿胎盘
血管生成
基因表达
Dazu Black goat
early pregnancy
fetal placenta
angiogenesis
gene expression
