摘要
背景:目前建立VX2皮下肿瘤模型的方法有多种,但建模方法各有优劣,能优质高效建立动物肿瘤模型的方法方可满足动物肿瘤实验的需求。目的:对比观察不同方法建立兔VX2皮下肿瘤模型,探索更简单高效的建模方式,为兔VX2皮下肿瘤实验提供大批量高质量的动物模型奠定基础。方法:雄性新西兰大白兔66只。取2只制备VX2荷瘤种兔,再取荷瘤兔肿瘤组织分别制备瘤组织块及肿瘤组织悬液备用。实验分为2组:瘤组织悬液组20只,麻醉后于双侧后肢内侧面注射组织悬液0.15 mL;瘤组织块组20只,麻醉后于双侧后肢内侧面皮下植入瘤组织块。2组分别采用相应接种方式种植2只荷瘤种兔,各传5代。记录并比较2组肿瘤种植时间,采用二维及彩色多普勒超声观察肿瘤形态大小及生长情况,比较2组肿瘤成瘤率及传代情况。实验方案经中国人民解放军第三军医大学动物实验伦理委员会批准(批准号为AMUWE2020016)。结果与结论:①组织悬液法肿瘤种植时间(75.70±11.16)s较组织块法肿瘤种植时间(100.80±9.21)s明显缩短(P=0.00);②组织悬液法成瘤率95%显著高于组织块法成瘤率60%(P<0.05),肿瘤体积显著大于组织块法(P<0.05);③组织悬液法传代成功率95%显著高于组织块法传代成功率65%(P<0.05);④结果说明,组织悬液法建立兔VX2皮下肿瘤模型与瘤块法相比,更简单高效。
BACKGROUND:There are many methods to establish VX2 subcutaneous tumor model,and the existing modeling methods have their own advantages and disadvantages.To meet the needs of tumor experiments on animals,the method of establishing an animal tumor model with high-quality and high-efficiency is necessary.OBJECTIVE:To explore more simple and efficient way of modeling,provide rabbit VX2 subcutaneous tumorexperiment with large quantities of high-quality animal models by comparing different methods to establish rabbit VX2 subcutaneous tumor model.METHODS:Sixty-six male New Zealand white rabbits were enrolled.Two of the 66 rabbits were used to prepare VX2 tumor-bearing rabbits,and tumor tissues were taken from the tumor-bearing rabbits to prepare tumor tissue blocks and tumor tissue suspensions.There were two groups in the experiment.In tumor tissue suspension group(n=20),the rabbits were injected with 0.15 m L of tissue suspension on the medial side of bilateral hind limbs after anesthesia;in tumor tissue block group(n=20),tumor tissue blocks were implanted subcutaneously on the medial side of bilateral hind limbs after anesthesia.Two tumor-bearing rabbits from each group were subjected to the corresponding vaccination methods,each passed for five generations.Tumor inoculation time in the two groups was record and compared.The tumor size and growth were observed by ultrasound with 2-D and CDFI mode.Tumor-take rate and serial passage of tumor tissues were observed and compared between the two groups.The experimental protocol was approved by the Animal Experiment Ethics Committee of the Army Medical University of PLA(approval No.AMUWE2020016)RESULTS AND CONCLUSION:The tumor inoculation time in the tissue suspension group[(75.70±11.16)s]was significant shortened compared with that in the tissue block group[(100.80±9.21)s;P=0.00].The tumor-take rate was significantly higher in the tissue suspension group than the tissue block group(95%vs.60%;P<0.05).The tumor size was significantly larger in the tissue suspension group than the tissue block group(P<0.05).The rate of tumor tissue series passage was significantly higher in the tissue suspension group than the tissue block group(95%vs.65%;P<0.05).Therefore,tissue suspension method for making the model of rabbit VX2 subcutaneous tumor is simpler and more efficient compared with the tissue block method.
作者
刘娅
冯爽
益磋
余堰澜
刘政
Liu Ya;Feng Shuang;Yi Cuo;Yu Yanlan;Liu Zheng(Department of Ultrasound,Xinqiao Hospital,Army Medical University,Chongqing 400037,China)
出处
《中国组织工程研究》
CAS
北大核心
2020年第32期5173-5178,共6页
Chinese Journal of Tissue Engineering Research
基金
国家重点研发计划项目(2017YFC0107300),项目负责人:刘政。
关键词
组织悬液
组织块
兔
VX2
肿瘤模型
超声
成瘤率
tumor suspension
tissue block
rabbit
VX2
tumor model
ultrasound
tumor-take rate
