摘要
目的:探讨胃癌患者血清中miR-133a和DKK-1水平与胃癌病理类型及临床预后的相关性。方法:选取经病理检查确诊为胃癌患者84例,分析不同病理分型、病理分期、有无淋巴结转移及不同细胞分化程度下血清中miR-133a、DKK-1水平。结果:miR-133a在乳头状腺癌、黏液腺癌、低分化腺癌及腺鳞癌患者中显著降低(P<0.05),DKK-1在乳头状腺癌、黏液腺癌、低分化腺癌及腺鳞癌患者中均显著升高(P<0.05);TNM分期Ⅳ期中DKK-1最高、miR-133a最低,TNM分期Ⅰ期中DKK-1最低、miR-133a最高,DKK-1随TNM分期加重显著上升(P<0.05),而miR-133a随TNM分期增加而显著下降(P<0.05);淋巴结转移胃癌患者中的DKK-1显著高于无淋巴结转移的胃癌患者(P<0.05),淋巴结转移的胃癌患者中miR-133a显著低于无淋巴结转移的胃癌患者(P<0.05);不同细胞分化胃癌患者中低分化DKK-1水平最高、miR-133a水平最低,高分化DKK-1水平最低、miR-133a水平最高,DKK-1水平随T细胞分化程度增强显著下降(P<0.05),miR-133a水平随细胞分化程度增强显著上升(P<0.05);DKK-1及miR-133a在不同预后结局中的表达存在差异,胃癌死亡患者DKK-1水平高于存活超过2年的胃癌患者(P<0.05),胃癌死亡患者miR-133a水平低于存活超过时间2年的胃癌患者(P<0.05)。结论:胃癌患者血清中miR-133a、DKK-1水平与胃癌患者的病理分期、病理分型、有无转移及细胞分化程度之间有着密切联系,可以通过检测血清中miR-133a、DKK-1水平对胃癌患者疾病的进展情况进行初步诊断。
Objective:To investigate the correlation of pathological types and clinical prognosis of gastric cancer with serum miR-133 a and DKK-1.Methods:84 patients with gastric cancer confirmed by pathological examination were selected to analyze the levels of miR-133 a and DKK-1 in serum of patients with gastric cancer under different pathological types,pathological stages,lymph node metastasis and cell differentiation.Results:MiR-133 a was significantly decreased significantly in patients with papillary adenocarcinoma,mucinous adenocarcinoma,poorly differentiated adenocarcinoma and adenosquamous carcinoma,and DKK-1 was significantly increased in patients with papillary adenocarcinoma,mucinous adenocarcinoma,poorly differentiated adenocarcinoma and adenosquamous carcinoma.DKK-1 was the highest and miR-133 a was the lowest in TNM stage Ⅵ,and DKK-1 was the lowest and miR-133 a was the highest in TNM stage Ⅰ.DKK-1 was increased significantly with TNM stage aggravation,while miR-133 a was decreased significantly with TNM stage aggravation.DKK-1 in patients with lymph node metastasis was significantly higher than that in patients without lymph node metastasis,and miR-133 a in patients with lymph node metastasis was significantly lower than that in patients without lymph node metastasis.In patients with poorly differentiated gastric cancer,the level of DKK-1 was highest,and the level of miR-133 a was lowest.In patients with highly differentiated gastric cancer,the level of DKK-1 was lowest,and the level of miR-133 a was highest.The level of DKK-1 was decreased significantly with the increase of T cell differentiation,and the level of miR-133 a was increased significantly with the increase of T cell differentiation.The expression of DKK-1 and miR-133 a in different prognosis outcome was different,and the level of DKK-1 in dead gastric cancer patients was higher than that of gastric cancer pa-tients who survived for more than 2 years,while the level of miR-133 a in dead gastric cancer patients was lower than that of gastric cancer patients who survived for more than 2 years.Conclusion:The levels of miR-133 a and DKK-1 in the serum of patients with gastric cancer are closely related to the pathological stage,pathological classification,lymph node metastasis and cell differentiation degree of patients with gastric cancer.It can be preliminarily determined by detecting the levels of miR-133 a and DKK-1 in the serum of patients with gastric cancer.
作者
刘维帅
郭晓宁
杨洋
张娟娣
LIU Weishuai;GUO Xiaoning;YANG Yang(Department of Pathology,Yangling Demonstration Zone Hospital,Shaanxi Province,Yangling 712100)
出处
《陕西医学杂志》
CAS
2020年第6期751-754,共4页
Shaanxi Medical Journal
