摘要
目的:对白鲜皮粗多糖(DDP)进行分离纯化,并研究其抗银屑病作用。方法:DDP经膜分离技术截留相对分子质量小于10 kDa的组分(DDP-UF),采用DEAE-52纤维素柱分离纯化得到4种组分(DDP-UF-1,DDP-UF-2,DDP-UF-3,DDPUF-4);并通过红外光谱法,高效凝胶渗透色谱法(HPGPC),高效液相色谱法(HPLC),扫描电镜(SEM)测定其理化性质及结构特征。选用咪喹莫特乳膏诱导银屑病小鼠模型,己烯雌酚诱导雌鼠阴道上皮细胞增殖,酶联免疫吸附测定法(ELISA)检测各组小鼠血清白细胞介素(IL)-17与IL-23含量,苏木精-伊红(HE)染色法观察小鼠背部皮肤组织病理变化、阴道上皮细胞有丝分裂指数变化。结果:DDP-UF-1~4均具有多糖特征吸收峰,DDP-UF-1~4相对分子质量分别为10948,40148,32222,19943 Da;单糖组成及摩尔比分别为甘露糖-葡萄糖-半乳糖(32.45∶11.35∶8.69),甘露糖-鼠李糖-葡萄糖醛酸-葡萄糖-木糖(25.68∶23.44∶21.62∶18.86∶3.68),甘露糖-鼠李糖-葡萄糖醛酸-半乳糖醛酸-木糖-半乳糖(18.68∶4.61∶3.89∶1.65∶5.36∶6.21),葡萄糖醛酸-半乳糖醛酸-葡萄糖-木糖-半乳糖(11.63∶15.26∶5.32∶2.08∶3.46);扫描电镜(SEM)显示DDP-UF-1~4形态结构为片状或海绵状结构。DDP-UF-1与DDP-UF-3可改善银屑病小鼠背部皮损状态、抑制雌鼠阴道上皮细胞有丝分裂、明显降低血清IL-17,IL-23含量(P<0.05,P<0.01)。结论:DDP-UF-1与DDP-UF-3均具有良好的抗银屑病作用,其作用可能与抑制IL-23/IL-17信号通路有关。
Objective:To extract crude polysaccharides from Dictamnus dasycarpus(DDP)for separation and purification,and study its anti-psoriasis effect.Method:After interception of DDP with a molecular weight of less than 10 kDa(DDP-UF)using membrane separation technology,four components(DDP-UF-1,DDP-UF-2,DDP-UF-3,DDP-UF-4)were isolated and purified by DEAE-52 cellulose column.Then,the physical and chemical properties and structural characteristics of DDP-UF-1-4 samples were determined by infrared spectroscopy,high performance gel permeability chromatography(HPGPC),high performance liquid chromatography(HPLC)and scanning electron microscope(SEM).Imiquimod cream was selected to induce mouse models of psoriasis,diethylstilbestrol was used to induce vaginal epithelial cell proliferation in female mice,interleulein-17(IL-17)and IL-23 contents of serum in each mouse group were detected by enzyme-linked immunosorbent assay(ELISA),and skin tissues of the mouse back and vaginal epithelial cells had mitotic index changes by hematoxylin-eosin staining(HE).Result:DDP-UF-1-4 all exhibited the characteristic absorption peaks of polysaccharide,and the molecular weights of DDP-UF-1-4 were 10948,40148,32222 and 19943 Da,respectively.The monosaccharide compositions and mole ratios of DDP-UF-1-4 were mannose-glucose-galactose(32.45∶11.35∶8.69),mannoserhamnose-glucuronic acid-glucose-xylose(25.68∶23.44∶21.62∶18.86∶3.68),mannose-rhamnose-glucuronic acid-galacturonic acid-xylose-galactose(18.68∶4.61∶3.89∶1.65∶5.36∶6.21),glucuronic acid-galacturonic acid-glucose-xylose-galactose(11.63∶15.26∶5.32∶2.08∶3.46),respectively.SEM showed that the morphological structures of DDP-UF-1-4 were flaky or spongy.The drug groups of DDP-UF-1 and DDP-UF-3 improved the skin condition of the psoriasis mice back,inhibited mitosis of female vaginal epithelial cells and significantly reduced the contents of IL-17 and IL-23 in serum(P<0.05,P<0.01).Conclusion:Both DDP-UF-1 and DDP-UF-3 have good anti-psoriasis effects,which may be related to the inhibition of IL-23/IL-17 signaling pathway.
作者
张凯
赵宏
张宇
王丽红
沈宇
王宇亮
ZHANG Kai;ZHAO Hong;ZHANG Yu;WANG Li-hong;SHEN Yu;WANG Yu-liang(School of Pharmacy,Jiamusi University,Jiamusi 154007,China;School of Pharmacy,Heilongjiang University of Chinese Medicine,Harbin 150040,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2020年第9期157-166,共10页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81603282)
中国博士后科学基金面上项目(2015M571454)
黑龙江省自然科学基金青年项目(QC2018119)
黑龙江省博士后科研基金项目(LBH-Z14204)
黑龙江省教育厅青年创新人才项目(UNPYSCT-2016095)
大学生创新创业训练计划项目(2018xj10)
黑龙江省教育厅基本科研业务费基础研究项目(2017-KYYWF-0585)
黑龙江省北药与功能食品优势特色学科建设项目。
关键词
白鲜皮
多糖
银屑病
炎症因子
小鼠阴道上皮细胞
Dictamnus dasycarpus
polysaccharides
psoriasis
inflammatory factor
mouse vaginal epithelium
