摘要
目的:探讨各级别及复发胶质瘤中miRNA-200c(miR-200c)的表达及可能机制。方法:收集本院收治的43例胶质瘤手术患者肿瘤切除标本,按照组织学分级不同将43例胶质瘤手术患者分为低级别胶质瘤组15例、高级别胶质瘤组22例、复发胶质瘤组6例,另选取10例正常脑组织标本为对照组。使用RT-PCR检测各级别初发胶质瘤及复发胶质瘤患者肿瘤组织中miR-200c的表达水平,通过Western blotting检测肿瘤干性指标(CD133、Nestin)及上皮间充质转化(EMT)标志物(E-cadherin、N-cadherin和Vimentin)在各组织中的表达。结果:各组miR-200c表达水平随着胶质瘤级别升高逐渐降低,两两组间比较差异均有统计学意义(P<0.05);各组Nestin表达水平随着胶质瘤级别升高逐渐升高,E-cadherin表达水平随着胶质瘤级别升高逐渐降低,两两组间比较差异均有统计学意义(P<0.05);高级别胶质瘤组、复发胶质瘤组CD133表达水平均较对照组、低级别胶质瘤组显著升高(P<0.05);高级别胶质瘤组、复发胶质瘤组N-cadherin、Vimentin表达水平均较对照组显著升高(P<0.05),且复发胶质瘤组N-cadherin、Vimentin表达水平均较低级别胶质瘤组、高级别胶质瘤组显著升高(P<0.05)。结论:miR-200c在复发及高级别胶质瘤中表达下降,其缺失可能诱导EMT使肿瘤干性增加,可作为高级别及复发胶质瘤治疗的潜在靶点。
Objective:To investigate the expression and possible mechanism of miRNA-200c(miR-200c)in different grades of glioma and recurrent glioma.Method:The tumor resection specimens of 43 patients with glioma were collected,according to the histological classification,43 patients were divided into low-grade glioma group(15 cases),high-grade glioma group(22 cases)and recurrent glioma group(6 cases),another 10 normal brain tissues were selected as the control group.RT-PCR was used to detect the expression of miR-200c in primary and recurrent glioma,Western blotting was used to detect the expression of tumor dryness index(CD133,Nestin)and epithelial mesenchymal transition(EMT)markers(E-cadherin,N-cadherin,Vimentin)in various tissues.Result:The miR-200c expression level of each group decreased with the increase of glioma grade,the differences between the two groups were statistically significant(P<0.05).The Nestin expression level of each group increased with the increase of glioma grade,the E-cadherin expression level of each group decreased with the increase of glioma grade,the differences between the two groups were statistically significant(P<0.05).The expression level of CD133 in high-grade glioma group and recurrent glioma group were significantly higher than those in control group and low-grade glioma group(P<0.05).The expression levels of N-cadherin and Vimentin in high-grade glioma group and recurrent glioma group were significantly higher than those in the control group(P<0.05),and the expression levels of N-cadherin and Vimentin in the recurrent glioma group were significantly higher than those in the low-grade glioma group and the high-grade glioma group(P<0.05).Conclusion:The expression of miR-200c decreased in recurrent and high-grade glioma,and the absence of miR-200c may induce EMT to increase tumor dryness,which may be a potential target for the treatment of high-grade and recurrent glioma.
作者
卢恒聪
李镇宇
邓敏峰
曹志恺
LU Hengcong;LI Zhenyu;DENG Minfeng;CAO Zhikai(Guangzhou First People's Hospital,Guangzhou 510180,China)
出处
《中国医学创新》
CAS
2020年第12期1-5,共5页
Medical Innovation of China
基金
广州市医药卫生科技项目(20171A011238)。
