摘要
【目的】研究白虎汤对实验性急性热应激小鼠的保护作用。【方法】采用生化培养箱法对小鼠进行热处理,热处理前先给小鼠灌胃高低2个剂量的白虎汤,试验结束时采集小鼠血液、肝脏和空肠组织,检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、肌酸激酶(CK)、尿素(UREA)含量以及肝组织中丙二醛(MDA)、谷胱甘肽(GSH)含量和总超氧化物歧化酶(T-SOD)活性,观察肝脏组织与空肠组织病理学变化。【结果】热处理后的小鼠体重显著减轻(P<0.05),热处理对照组小鼠血清ALT、AST、CK和UREA含量显著升高(P<0.05),肝组织GSH含量和T-SOD活性显著降低(P<0.05),MDA含量显著升高(P<0.05),肝组织呈现大量中央静脉淤血和肝细胞不规则排列;白虎汤高剂量组小鼠血清ALT、AST和UREA水平显著降低,肝组织GSH含量和T-SOD活性升高,肝组织中央静脉淤血减少并且肝细胞排列较整齐。【结论】白虎汤对实验性急性热应激小鼠有较好的保护作用,可以有效缓解热应激造成的小鼠肝组织损伤。
[Purpose]To investigate the protective effects of Baihu decoction on the experimental acute heat stress of mice.[Method]The mice were heat-treated by biochemical incubator method.Before the heat-treatment,the mice were given two doses of Baihu decoction.At the end of the experiment,the blood and liver and jejunum tissues of the mice were collected to detect the levels of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),creatine kinase(CK)and UREA,content of liver tissue malondialdehyde(MDA)and glutathione(GSH)and activity of total superoxide dismutase(T-SOD).The pathological changes of liver tissue and jejunum were observed.[Result]After heat-treatment,the weight of mice was significantly reduced(P<0.05).Serum ALT,AST,CK and UREA levels of the heat stress control group were significantly increased(P<0.05),GSH content and T-SOD activity in liver tissues were significantly decreased(P<0.05),and MDA content was significant increased(P<0.05);the major lesions in liver tissue were central venous congestion and irregular arrangement of liver cells.Serum levels of ALT,AST and UREA in Baihu de-coction high dose group were significantly decreased,GSH content and T-SOD activity of liver tissue were increased,and the central venous congestion of the liver tissue was reduced with liver cells arranged neatly.[Conclusion]Baihu decoction has a good protective effect on experimental acute heat stress mice,and can effectively alleviate liver tissue damage caused by heat stress in mice.
作者
王思思
韩春杨
徐光沛
张梦迪
刘翠艳
WANG Sisi;HAN Chunyang;XU Guangpei;ZHANG Mengdi;LIU Cuiyan(College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,China)
出处
《云南农业大学学报（自然科学版）》
CSCD
北大核心
2020年第2期289-294,共6页
Journal of Yunnan Agricultural University:Natural Science
基金
安徽省重点研究与开发计划项目(1704a07020072)
安徽农业大学2018年校级质量工程项目(2018aujyssf01)。
关键词
白虎汤
急性热应激
小鼠
保护作用
Baihu decoction
acute heat stress
mice
protective effect
