摘要
采用膨胀颗粒污泥床(EGSB)和上升式厌氧污泥床(UASB)反应器在不同运行条件下培养厌氧氨氧化颗粒污泥,对比分析颗粒污泥性质和微生物群落的差异性.研究表明接种厌氧氨氧化絮状污泥经过EGSB和UASB反应器运行384 d后,均能实现颗粒化,颗粒污泥平均粒径分别达到1.17 mm和1.21 mm,各范围(<0.2、 0.2~1.5、 1.5~3和>3 mm)的粒径占比为6.06%、 60.05%、 25.25%和8.64%, 7.40%、 58.90%、 32.04%和1.66%.扫描电镜结果表明不同运行条件下的污泥菌群均以短杆菌、球型菌为主.高通量测序结果表明,Shannon指数EGSB反应器为7.52高于UASB反应器为7.18;变形菌门(Proteobacteria)是两个反应器各阶段污泥的主要菌门,浮霉菌门(Planctomycetes)从接种时的3.30%增到第384d的12.30%(EGSB)和13.30%(UASB).EGSB反应器中的主要厌氧氨氧化菌属为Candidatus Brocadia占7.53%,其次为Candidatus Kuenenia属占1.61%;而在UASB反应器中Candidatus Brocadia属和Candidatus Kuenenia属分别占比为3.69%和7.54%,Candidatus Kuenenia是其优势厌氧氨氧化菌属.优势菌群丰度与环境因子变化存在联系,Candidatus Brocadia丰度与上升流速(v)、氮容积去除负荷(NRR)呈正相关而与水力停留时间(HRT)呈负相关,Candidatus Kuenenia与氮负荷去除率(NRE)、NRR、HRT呈正相关,而与v呈负相关.
Anaerobic ammonium oxidation(ANAMMOX) granular sludge was cultured during different operating conditions by an expanded granular sludge bed(EGSB) reactor and up-flow anaerobic sludge bed(UASB) reactors, and the characteristics of the granular sludge and microbial community were compared. The results showed that the flocculent ANAMMOX sludge can be granulated after being operated for 384 days by the EGSB and UASB reactors. The average particle size reached 1.17 mm and 1.21 mm, respectively. The particle size ratio of each range(<0.2, 0.2-1.5, 1.5-3, and>3 mm) was 6.06%, 60.05%, 25.25%, and 8.64% in the EGSB reactor, and 7.40%, 58.90%, 32.04%, and 1.66% in the UASB reactor, respectively. The results of scanning electron microscopy showed that the bacterial flora during different operating conditions were mainly Brevibacterium and Cocci aggregates. High-throughput sequencing results showed that the Shannon index of the EGSB reactor was 7.52, higher than the 7.18 of the UASB reactor on day 384;Proteobacteria was the main phylum of the sludge at each stage, and Planctomycetes increased from 3.30% to 12.30% in the EGSB reactor and 13.30% in the UASB reactor on day 384. The main ANAMMOX genera in the EGSB reactor were Candidatus Brocadia, accounting for 7.53%, followed by Candidatus Kuenenia accounting for 1.61%, whereas in the UASB reactor, Candidatus Kuenenia was the dominant anaerobic ammonia genus, accounting for 7.54%, followed by Candidatus Brocadia, which accounted for 3.69%. The proportion of dominant species was related to the change in environmental factors. The proportion of Candidatus Brocadia was positively correlated with the up-flow rate and nitrogen removal rate(NRR), but negatively correlated with hydraulic retention time(HRT). Candidatus Kuenenia was positively correlated with nitrogen removal efficiency(NRE), NRR, and HRT, but negatively correlated with the up-flow rate.
作者
姜滢
郭萌蕾
谢军祥
常尧枫
谢嘉玮
陈重军
沈耀良
JIANG Ying;GUO Meng-lei;XIE Jun-xiang;CHANG Yao-feng;XIE Jia-wei;CHEN Chong-jun;SHEN Yao-liang(School of Environmental Science and Engineering,Suzhou University of Science and Technology,Suzhou 215009,China;Jiangsu Collaborative Innovation Center of Water Treatment Technology and Material,Suzhou 215009,China;Jiangsu Provincial Key Laboratory of Environmental Science and Engineering,Suzhou 215009,China)
出处
《环境科学》
EI
CAS
CSCD
北大核心
2020年第5期2358-2366,共9页
Environmental Science
基金
国家自然科学基金项目(51508366)
苏州市民生科技项目(ss2019022)
江苏水处理技术与材料协同创新中心预研项目(XTCXSZ2019-3)
江苏省环境科学与工程重点实验室开放基金项目(Zd1804)。
关键词
培养条件
厌氧氨氧化
颗粒污泥性质
微生物群落结构
culture conditions
anaerobic ammonium oxidation
granular sludge properties
microbial community structure
