摘要
从河北省番茄栽培面积较大的定兴和肥乡地区枯萎病病株中分离纯化菌株,通过形态学和分子生物学等方法对病原菌进行鉴定,并采用浸根法鉴定了10个番茄砧木的枯萎病抗性。结果表明,从定兴和肥乡分离到的菌株均为尖孢镰孢菌,采用尖孢镰孢菌生理小种特异性引物进行PCR扩增,证明2个菌株都为尖孢镰孢菌番茄专化型(Fusarium oxysporum f.sp.lycopersici)生理小种3;筛选得到了3个枯萎病高抗砧木品种,7个抗病砧木品种。
This paper seperated and purified bacterial strains from wilt tomato plants grown in Dingxing and Feixiang counties with large tomato cultivation areas of Hebei Province.Mophology and molecular biology methods were adopted to identify the pathogenic bacteria.The wilt resistance of 10 tomato rootstocks were identified by root dip method.The results showed that both strains isolated from Dingxing and Feixiang belonged to Fusarium oxysporum f.sp.lycopersici.PCR amplification was conducted using specificity primers of Fusarium oxysporum f.sp.lycopersici race,It was proved that these 2 bacterial strains were specialized forms of Fusarium oxysporum f.sp.lycopersici race 3.Three high resistant and 7 resistant rootstock varieties were screened out.
作者
褚新培
吕桂云
鹿秀云
贾邱颖
梁垚
高洪波
CHU Xin-pei;LYU Gui-yun;LU Xiu-yun;JIA Qiu-ying;LIANG Yao;GAO Hong-bo(College of Horticulture,Hebei Agricultural University,Collaborative Innovation Center of Vegetable Industry in Hebei,Baoding 071000,Hebei,China;Institute of Plant Protection,Hebei Academy of Agricultural and Forestry Sciences,Baoding 071001,Hebei,China)
出处
《中国蔬菜》
北大核心
2020年第6期64-68,共5页
China Vegetables
基金
河北省重点研发计划项目农业关键共性技术攻关专项(18226907D)
国家自然科学基金项目(31872132)。
关键词
番茄
枯萎病菌
砧木
抗病性
Tomato
Fusarium wilt
Rootstock
Disease resistance
