摘要
从受氮污染浅层含水层介质中分离纯化得到1株高效异养硝化-好氧反硝化细菌XK51,经过菌落形态、生理生化特性及16S rDNA基因序列分析,鉴定该菌株为假单胞菌属恶臭假单胞菌(Pseudomonas Putida)。脱氮性能结果表明:XK51为兼性反硝化细菌,能在好氧或缺厌氧条件下高效反硝化脱氮,最大和平均反硝化速率分别为27.3,4.4 mg/(L·h),硝酸盐脱除率为95.3%;该菌株同时具有较高异养硝化能力,最大和平均硝化速率分别为4.2,1.4 mg/(L·h),氨氮脱除率为98.5%。XK51最佳碳源为柠檬酸三钠,适宜生长温度为28~35℃,最适温度为30℃;适宜生长pH为6.5~8.0,最适pH为7.0。XK51可同时进行异养硝化及同步硝化-反硝化,培养期间未出现明显亚硝酸盐和硝酸盐累积,在含氮污废水处理和地下水氮污染修复方面具有潜在工程应用价值。
A heterotrophic nitrification and aerobic denitrification bacterium strain named XK51 was isolated and purified from nitrogen-contaminated shallow aquifer porous media.The strain was identified as Pseudomonas Putida by morphological observation,physiological and biochemical characteristics and 16S rDNA gene sequence analysis.The results showed that strain XK51 was a facultative denitrifying bacteria,which could achieve high denitrification efficiencies under aerobic or anaerobic conditions.The maximum and average denitrification rate were 27.3 mg/(L·h)and 4.4 mg/(L·h),the nitrate removal efficiency was 95.3%.XK51 also had high heterotrophic nitrification capacity,and its maximum and average nitrification rate were 4.2 mg/(L·h)and 1.4 mg/(L·h),then the ammonia removal efficiency was 98.5%.The optimum carbon source was trisodium citrate,and the optimum growth temperature ranged from 28 to 35℃,with a preferred value of 30℃.The optimum cultivating pH value ranged from 6.5 to 8.0,with a preferred value of 7.0.Strain XK51 could simultaneously carry out heterotrophic nitrification and simultaneous nitrification-denitrification with no obvious nitrite and nitrate accumulation during cultivation.It has potential engineering application value in nitrogen-containing wastewater treatment and nitrogen pollution remediation of groundwater.
作者
朱晓明
赵东华
阮晓红
ZHU Xiao-ming;ZHAO Dong-hua;RUAN Xiao-hong(Shanghai Waterway Engineering Design and Consulting Co.,Ltd,Shanghai 200120,China;School of Earth Sciences and Engineering,Nanjing University,Nanjing 210093,China)
出处
《环境工程》
CAS
CSCD
北大核心
2020年第5期43-48,共6页
Environmental Engineering
基金
国家自然科学基金项目(41230640,41807187)
中交上航局科研项目(2017-094J)
中交集团科研项目(2019-268J)。
关键词
浅层含水层介质
异养硝化
好氧反硝化
分离鉴定
脱氮性能
unconfined aquifer porous media
heterotrophic nitrification
aerobic denitrification
isolation and identification
nitrogen removal characteristics
