摘要
提出一种基于LED角度照明优化模式的傅里叶叠层显微成像方法。首先,根据LED、孔径和样品之间的关系,获取傅里叶平面可扩展的理论频谱范围;其次,使用图像质量评价指标衡量不同照明方式下的重建图像质量差异,构造任意单个LED对整体重建结果影响的差异函数;然后,通过对差异表达式的分析与仿真,制定了最佳的角度照明策略,设计出一种基于菱形采样方法来加速傅里叶叠层显微成像的实现过程;最后,采用主观和客观的评价指标对仿真和实验的有效性进行评估。结果表明,本文方法在保持重建质量的前提下有效提高了成像效率,其效率可提高到传统方法的3.85倍。
In this study,an efficient Fourier ptychographic microscopy method(FPM)based on an optimized pattern of light emitting diode(LED)angle illumination is proposed.First,a theoretical expandable spectrum range is obtained in the Fourier space based on the relationship among the LED,the aperture,and the sample.Second,image quality assessment methods are utilized to extract differential expressions to describe how an arbitrary LED affects the reconstruction effect.Third,the optimized angle illumination strategy is implemented based on the analysis of the differential expression.Then,a rhombus-based sampling approach is designed to accelerate the FPM process.Finally,the subjective and objective assessments are used to measure the validity of both simulation and actual experiments.Results indicate that our optimized method can effectively improve the efficiency by approximately 3.85 times compared with that of the traditional method.
作者
李通
赵巨峰
毛海锋
崔光茫
胡金星
Li Tong;Zhao Jufeng;Mao Haifeng;Cui Guangmang;Hu Jinxing(School of Electronics and Information,Hangzhou Dianzi University,Hangzhou,Zhejiang 310018,China;HwaMei Hospital,University of Chinese Academy of Sciences,Ningbo,Zhejiang 315010,China)
出处
《激光与光电子学进展》
CSCD
北大核心
2020年第8期268-277,共10页
Laser & Optoelectronics Progress
基金
国家自然科学基金(61805063)
浙江省自然科学基金(LY18F050007)
浙江省医药卫生科技项目(2018KY697)
浙江省科协“育才工程”项目(SKX201901)。
关键词
成像系统
计算成像
傅里叶叠层显微
效率
菱形
imaging systems
computational imaging
Fourier ptychography microscopy
efficiency
rhombus
