摘要
目的 探讨藿香正气合剂对5 Gyγ射线照射诱导的小鼠辐射损伤的防护作用.方法 将6~8周龄的健康无特定病原体级C57BL/6J雄性小鼠按随机区组法分为正常对照组(n=10)、γ射线照射组(n=15)和γ射线照射+藿香正气合剂组(n=15).除正常对照组外,另2组小鼠给予5 Gyγ射线一次性全身照射后1小时内,γ射线照射+藿香正气合剂组小鼠给予200 μL藿香正气合剂灌胃,对照组和单纯照射组给予200 μL饮用水灌胃.每天给药1次,连续给药10d,每天记录小鼠的体重变化.10d后对小鼠进行摘眼球取血测定血常规各项指标,脱颈处死小鼠并称各脏器重量.采用t检验对组间数据进行比较.结果 γ射线照射的2组小鼠的体重低于正常对照组小鼠,在照射后第4、6、7、8、9和10天,γ射线照射+藿香正气合剂组小鼠的体重均高于γ射线照射组,且差异均有统计学意义(t=-2.138~2.529,P=-0.027~0.045).γ射线照射+藿香正气合剂组小鼠的心脏、肝脏、脾脏和胸腺的脏器指数均高于γ射线照射组,且差异均有统计学意义(t=1.768、1.894、2.085、1.992,P=-0.022、0.023、0.038、0.044).γ射线照射+藿香正气合剂组与γ射线照射组小鼠的脾脏和胸腺重量的差异最大,且均有统计学意义(t=2.517、2.158,P=0.028、0.029).3组小鼠血液中血红蛋白浓度、白细胞数量和血小板数量等多项血常规指标之间有差异,且均有统计学意义(t=2.262~3.916,P=0.000~0.005).结论 藿香正气合剂能减缓5 Gyγ射线照射诱导的小鼠体重降低和造血系统损伤,且有一定的辐射防护作用.
Objective To explore the in vivo effects of Huoxiang Zhengqi mixture on radiation injury induced by 5 Gyγirradiation in mice.Methods Male 6-to 8-week-old C57BL/6J mice at specific pathogen-free level were randomly categorized into the three following groups according to body mass:normal control(NC,n=10),ionizing radiation(IR,n=15),and IR+Huoxiang Zhengqi mixture groups(I+H,n=15).Except for the NC group,the two other groups were treated with single total body 5 Gyγirradiation.All three groups receive gavage administration of 200μL of either Huoxiang Zhengqi mixture(I+H group)or drinking water(NC and IR groups)within 1 h after irradiation.The administration was performed once a day for 10 days.Body weight was also measured.After the treatment period,blood was extracted from the eyeball and collected into heparin-treated tubes to examine routine blood indices before the mice were sacrificed from the neck.Major organ weights were measured,and organ coefficients were analyzed.Independent sample t test was used for the intergroup comparison.Results The body weights of the IR and I+H groups were lower than those in the NC group,and yet on the fourth,sixth,seventh,eighth,ninth and tenth days after irradiation,the body weights of the I+H group were higher than those in the IR group(t=2.138-2.529,P=0.027-0.045).The organ coefficients of heart,liver,thymus,and spleen in the I+H group were higher than those in the IR group,and the differences were statistically significant(t=1.768,1.894,2.085,1.992;P=0.022,0.023,0.038,0.044).The differences in the spleen and thymus weights between the IR and I+H groups were significant(t=2.517,2.158;P=0.025,0.029).Multiple blood indices such as hemoglobin,white blood cell count,and blood platelet between each group were significantly different(t=2.262-3.916,P=0.000-0.005).Conclusions Huoxiang Zhengqi mixture can alleviate the weight loss and hematopoietic system damage of mice induced byγ-ray irradiation(5 Gy),and exert certain radiation protection function.
作者
张书琴
崔明
王滨
樊赛军
Zhang Shuqin;Cui Ming;Wang Bin;Fan Saijun(Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine,Institute of Radiation Medicine,Chinese Academy of Medical Science,Peking Union Medical College,Tianjin 300192,China)
出处
《国际放射医学核医学杂志》
2020年第3期156-163,共8页
International Journal of Radiation Medicine and Nuclear Medicine
基金
国家自然科学基金(81803062、81572969)
中国医学科学院医学与健康科技创新工程"一带一路"战略先导科研专项(2017-I2M-B&R-13)
中国博士后科学基金(2018M631391)。
关键词
Γ射线
造血系统
辐射损伤
辐射防护
藿香正气合剂
Gamma rays
Hemopoietic system
Radiation injuries
Radiation protection
Huoxiang Zhengqi mixture
