摘要
目的探讨高灵敏度HCV RNA检测在术前筛查中的应用。方法收集2019年1月至10月间本院术前筛查患者血清标本1005例,同时采用化学发光微粒子免疫分析法(CMIA)和高灵敏度实时荧光定量PCR法分别检测血清中的HCV抗体和HCV RNA载量。对HCV抗体S/CO值与高灵敏度HCV RNA定量检测结果进行比较与相关性分析,并对HCV抗体S/CO值预测丙型肝炎病毒血症的效能进行评价。结果1005例血清标本中,HCV抗体阳性标本228例,阳性检出率为22.7%,未检出HCV抗体阴性而HCV RNA阳性标本;在HCV抗体阳性的样本中,高灵敏度实时荧光定量PCR法检测出HCV RNA阳性样本83例,检出率为36.4%;HCV抗体S/CO值的高低与HCV RNA定量值之间无相关性。以HCV抗体S/CO值≥10.7作为预测丙型肝炎病毒血症的临界值,其灵敏度与特异度分别为92.59%和77.93%,可较好地预测HCV病毒血症。结论高灵敏度HCV RNA定量检测用于术前筛查丙型肝炎患者,可以准确反映患者体内病毒的复制情况和传染性,与传统的术前筛查方法相比,可区分现症感染和既往感染,并具有快速、灵敏和特异的优点,有良好的临床应用价值。
Objective To investigate the application of high⁃sensitivity HCV RNA detection in preoperative screening.Methods A total of 1005 serum samples were collected from the preoperative screening patients in the Hospital,Sun Yat⁃sen University from January to October 2019.HCV antibodies and HCV RNA were detected using chemiluminescence microparticle immunoassay(CMIA)and fluorescence quantitative polymerase chain reaction(FQ⁃PCR).The comparison and correlation analysis between the S/CO value of HCV antibody and the high⁃sensitivity HCV RNA test results were performed.The predictive efficacy of HCV antibody S/CO value for hepatitis C viremia was evaluated.Results Among the 1005 serum samples,228 were positive for HCV antibody,and the positive rate was 22.7%.HCV RNA were not detected from HCV antibody⁃negative specimens.High⁃sensitivity FQ⁃PCR method detected 83 HCV RNA positive cases and the positive rate was 36.4%.There was no correlation between the HCV antibody S/CO value and the HCV RNA quantitative value.Using the critical S/CO value≥10.7 with a sensitivity and specificity of 92.59%and 77.93%respectively can reduce the false positive rate for predicting hepatitis C viremia.Conclusion High⁃sensitivity HCV RNA quantitative detection is applied for the preoperative screening of patients with hepatitis C,which can accurately reflect the virus replication and infectivity in patients.Compared with the traditional preoperative screening method,FQ⁃PCR can distinguish the current infection from the previous infection,and has the advantages of rapid,sensitive and specific,and good clinical application value.
作者
何宇婷
陈雪芳
陈培松
黄浩
余学高
黄彬
HE Yuting;CHEN Xuefang;CHEN Peisong;HUANG Hao;YU Xuegao;HUANG Bin(Department of Laboratory Medicine,The First Affiliated Hospital,Sun Yat⁃sen University,Guangzhou,Guangdong,China,510080;Nanfang College of Sun Yat⁃sen University,Guangzhou,Guangdong,China,510970)
出处
《分子诊断与治疗杂志》
2020年第4期419-422,共4页
Journal of Molecular Diagnostics and Therapy
基金
广东省自然科学基金(2014A030313143)。
