摘要
为揭示Cd胁迫下马铃薯根际土壤细菌群落结构差异及根际细菌对Cd胁迫的响应特征,并为深入研究马铃薯根际土壤细菌与耐Cd性之间的关系提供理论基础,应用高通量测序技术对不同含量(0、10、50、100 mg/kg)Cd胁迫下马铃薯根际土壤细菌多样性和群落结构进行研究。结果表明,Cd含量为100 mg/kg时,马铃薯根际土壤细菌多样性最低;Cd含量为10 mg/kg时,马铃薯根际土壤细菌多样性最高。在门水平上,变形菌门(Proteobacteria)为优势菌门,占比为37.78%~48.28%;在属水平上,水恒杆菌属(Mizugakiibacter)为优势菌属,占比为9.16%~20.44%。主成分分析表明,马铃薯根际土壤细菌受Cd胁迫后,其群落结构与对照具有明显差异。利用相关性分析得到3个对Cd具有耐性的细菌菌属,分别是苔藓杆菌属(Bryobacter)、Flexivirga和Jatrophihabitans。
In order to reveal the difference of bacterial community structure in rhizosphere soil and the change characteristics of rhizosphere bacteria under Cd stress,and understand the relationship between bacteria and Cd tolerance in potato rhizosphere soil,the bacterial diversity and community structure of potato rhizosphere soil under Cd stress with different concentrations(0,10,50,100 mg/kg)were studied by high throughput sequencing.The results showed that the bacterial diversity of potato rhizosphere soil was the lowest when the Cd stress concentration was 100 mg/kg,and the highest when the Cd stress concentration was 10 mg/kg.Proteobacteria was the dominant phylum at phyla level,accounting for 37.78%—48.28%.At genus level,Mizugakiibacter was dominant,accounting for 9.16%—20.44%.Principal component analysis showed that the community structure of bacteria in potato rhizosphere soil under Cd stress was significantly different from that of control.Three genera of Cd-tolerant bacteria,Brubacter,Flexivirga and Jatrophilhabitans,were identified by correlation analysis.
作者
谢朝
付天岭
何腾兵
成剑波
符东顺
罗胜
XIE Zhao;FU Tianling;HE Tengbing;CHENG Jianbo;FU Dongshun;LUO Sheng(College of Agriculture,Guizhou University,Guiyang 550025,China;Guizhou Province Mountain Livestock and Poultry Breeding Pollution Control and Resource Technology Engineering Laboratory,Guiyang 550025,China;Institute of New Rural Development,Guizhou University,Guiyang 550025,China;College of Life Sciences,Guizhou University,Guiyang 550025,China)
出处
《河南农业科学》
北大核心
2020年第6期48-58,共11页
Journal of Henan Agricultural Sciences
基金
国家自然科学基金委员会-贵州省人民政府喀斯特科学研究中心项目(U1612442)
贵州省生物学一流学科建设项目(GNYL[2017]009)。
关键词
CD胁迫
马铃薯
根际土壤
聚类分析
高通量测序
相对丰度
细菌群落多样性
耐性菌
Cd stress
Potato
Rhizosphere soil
Cluster analysis
High-throughput sequencing
Relative abundance
Bacterial community diversity
Tolerant bacterium
