摘要
用KH2PO4浓度为0.005mmol/L的Hoagland全营养液模拟低磷胁迫,对低磷环境下高羊茅的生长特性、生理响应以及基因组DNA甲基化进行分析,探讨高羊茅对低磷胁迫的响应。结果表明:低磷胁迫15d后高羊茅的植株生长受到抑制,分蘖数明显降低;地上部、地下部的鲜重和干重受低磷胁迫影响较大,生物量的分配更倾向于地下部;低磷胁迫对高羊茅叶片生理生化指标的影响不一致,叶片中可溶性蛋白(SP)含量显著降低(P<0.05),超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽还原酶(GR)活性显著降低(P<0.05),而过氧化物酶(POD)活性显著升高(P<0.05);低磷环境下玉米素(ZA)含量较对照无显著差异,而脱落酸(ABA)含量显著下降(P<0.05),赤霉素(GA3)和植物生长素(IAA)含量则显著升高(P<0.05),其中IAA相对变化率最大。甲基化敏感扩增多态性(MSAP)分析发现,与对照相比,低磷组总甲基化率下降2.46%,低磷组甲基化模式的变化主要以甲基化为主,磷胁迫诱导的高羊茅DNA甲基化变化可能参与了对环境的适应性调节。
To investigate the physiological response under low phosphorus stress,tall fescue(Festuca arundinacea Schreb.)was used as experimental materials.The growth characteristics,physiological response and genomic DNA methylation of tall fescue under low phosphorus stress were analyzed by using Hoagland nutrient solution with KH 2PO 4 concentration of 0.005mmol/L for 15 days to simulate low phosphorus stress.The results showed that the growth of tall fescue was inhibited and the tiller number was significantly reduced under low phosphorus stress;the fresh and dry weight of the aboveground and underground were greatly affected by low phosphorus stress,and the distribution of biomass under low phosphorus was more inclined to roots.The effect of low phosphorus stress on physiological and biochemical indexes of tall fescue leaves was inconsistent.The soluble protein(SP)content in leaves was significantly decreased(P<0.05).The superoxide dismutase(SOD),catalase(CAT)and glutathione reductase(GR)activity were significantly decreased(P<0.05),while peroxidase(POD)activity was significantly increased(P<0.05).The zeatin(ZA)contents were no significant difference compared with the control group(P<0.05),while the abscisic acid(ABA)contents decreased significantly under low phosphorus stress(P<0.05),the contents of gibberellin(GA 3)and IAA increased significantly(P<0.05),and the relative change rate of IAA was the largest.Methylation sensitive amplification polymorphism(MSAP)analysis showed that the total methylation rate of the low phosphorus group decreased by 2.46%compared with the control group.Further analysis of DNA methylation patterns showed that the methylation was mainly change of methylation pattern of the low phosphorus group.The DNA methylation of tall fescue induced by phosphorus stress may be involved in adaptive regulation of environment,which provides a reference for the discussion of response mechanism of tall fescue to low phosphorus.
作者
王子苑
舒健虹
吴佳海
陈莹
王小利
WANG Zi-yuan;SHU Jian-hong;WU Jia-hai;CHEN Ying;WANG Xiao-li(Guizhou Institute of Prataculture,Guiyang 550006,China)
出处
《中国草地学报》
CSCD
北大核心
2020年第3期7-14,共8页
Chinese Journal of Grassland
基金
贵州省高层次创新人才(黔科合平台人才〔2018〕5634)。
关键词
高羊茅
低磷胁迫
生理响应
DNA甲基化
Tall fescue
Low phosphorus stress
Physiological response
DNA methylation
