摘要
目的研究黄金血康胶囊主要功效成分鉴别及测定其总黄酮含量。方法样品经溶剂提取、分离及纯化采用薄层色谱法(thin layer chromatography,TLC)、高效液相色谱法(high performance liquid chromatography,HPLC)及超高效液相色谱法(ultra performance liquid chromatography,UPLC)鉴别黄金血康胶囊主要功效成分及总黄酮含量测定。结果以洛伐他汀为对照品,薄层色谱法和超高效液相色谱法有效地鉴别了红曲粉。以丹参酮ⅡA为对照品,薄层色谱法有效地鉴别了丹参提取物。以芦丁为对照品,薄层色谱法有效地鉴别了银杏叶提取物。以槲皮素、山奈素及异鼠李素为对照品,高效液相色谱法测定了样品中总黄酮含量。其中槲皮素的回归方程Y=403X+337,r=0.9991,线性范围0~60μg/mL,方法检测限3 ng/mL,精密度为1.25%,回收率为99.1%。山奈素及异鼠李素这些参数与槲皮素基本相同。3批产品总黄酮含量重复性测定的相对标准偏差(relative standard deviation,RSD)分别为1.49%、2.05%、2.31%,符合定量测定要求。结论上述的鉴别和含量测定方法专属性强,重复性好,灵敏度高,可管控产品质量。
Objective To investigate the main functional ingredients and to determine the total flavonoids content in Gold blood kang capsule.Methods After the samples were extracted,separated and purified by solvent,the main functional ingredients were identified and total flavonoids content were determined by thin layer chromatography(TLC),high performance liquid chromatography(HPLC)and ultra performance liquid chromatography(UPLC)in Gold blood kang capsule.Results The monascus powder was effectively identified by TLC and UPLC using lovastatin as reference substance;the extract of Salvia miltiorrhiza Bunge was identified by TLC using tanshinoneⅡA as reference substance;the extract of ginkgo leaf was identified by TLC using rutin as reference substance;the flavonoids content was determined by HPLC,using quercetin,kaempferide and isorhamnetin as reference substances.Meanwhile,the regression equation of quercetin was Y=403X+337,r=0.9991.The range of linearity was 0~60μg/mL,limit of detection was 3 ng/mL,precision was 1.25%,and the recovery rate was 99.1%.The quantitative analysis of kaempferide and isorhamnetin were the same as quercetin.The relative standard deviation(RSD)of 3 batches of products was 1.49%,2.05%and 2.31%respectively,which conformed to the quantitative assay requirements.Conclusion The above methods for identification and content determination are strong specificity,good repeatability and high sensitivity,and can control the product quality.
作者
张连龙
刁春霞
周华生
成恒嵩
任会娜
高伟
ZHANG Lian-Long;DIAO Chun-Xia;ZHOU Hua-Sheng;CHENG Heng-Song;REN Hui-Na;GAO Wei(Wuxi Giant Pharmaceutical Co.Ltd.,Wuxi 214091,China)
出处
《食品安全质量检测学报》
CAS
2020年第8期2621-2629,共9页
Journal of Food Safety and Quality
关键词
黄金血康
保健食品
功效成分
鉴别
总黄酮含量
Gold blood kang
health food
functional ingredients
identification
total flavonoids
