摘要
目的:探讨3周有氧运动对慢性疲劳综合征大鼠海马区神经元形态结构及ERK/CREB/BDNF信号通路的影响。方法:雄性54只SD大鼠,随机分为3周对照组(C组)27只、模型组(M组)27只,C组常规饲养,M组进行三周慢性疲劳综合症(CFS)建模。建模结束后,C组、M组随机各选取6只进行取材;剩余模型组大鼠随机分为模型对照组(MC组)、模型运动组(ME组);剩余对照组随机分为6周对照组(CC组)、单纯运动组(CE组)。采用力竭游泳、束缚、禁食禁水、睡眠剥夺4种应激因素建立CFS模型,采用无负重游泳进行有氧运动干预。采用HE染色切片观察各组大鼠海马区神经元形态结构,采用Western Blotting方法检测信号通路P-ERK、CREB、P-CREB、BDNF蛋白的表达。结果:1)M组大鼠一般状况有明显的CFS特征;2)与C组相比,M组大鼠海马CA1区细胞损伤不明显,CA3区、DG区细胞出现明显损伤;MC组大鼠海马CA1区存在细胞损伤现象,CA3区、DG区细胞损伤现象有所好转;ME组、CC组、CE组大鼠细胞形态结构正常;3)与C组相比,M组大鼠海马区P-ERK蛋白表达显著下降(P<0.05),CREB、BDNF蛋白表达非常显著性下降(P<0.01);P-CREB蛋白表达没有显著性差异(P>0.05);4)与CC组相比,CE组大鼠海马组织P-ERK、CREB、P-CREB、BDNF蛋白表达均非常显著性增加(P<0.01);MC组大鼠海马组织P-ERK、CREB蛋白表达均非常显著性增加(P<0.01),P-CREB、BDNF蛋白表达均非常显著性减小(P<0.01);与CE组相比,ME组大鼠海马组织P-ERK蛋白表达非常显著性增高(P<0.01),CREB、P-CREB、BDNF蛋白表达均非常显著性减少(P<0.01)。结论:慢性疲劳综合征引起大鼠海马神经元损伤可能与ERK/CREB/BDNF信号通路有直接关系;有氧运动可促进大鼠海马ERK/CREB/BDNF信号通路蛋白的表达进而修复海马神经元损伤。
Objective:To investigate the effect of 3-week aerobic exercise on neuronal morphological structure and ERK/CREB/BDNF signaling pathway in hippocampus of chronic fatigue syndrome rats.Methods:54 male SD(Sprague-Dawley)rats were randomly divided into a 3-week control group(C)of 27 rats,and a model group(M)of 27 rats.After the modeling,6 samples were randomly selected from C group and M group for sampling.The remaining model groups were randomly divided into model control group(MC)and model exercise group(ME).The remaining control group was randomly divided into six-week control group(CC)and simple exercise group(CE).The CFS model was established based on four stress factors:exhaustive swimming,restraint,fasting and water deprivation,and sleep deprivation.Aerobic exercise intervention was performed by swimming without weight.HE staining sections were used to observe the morphological structure of neurons in the hippocampal area of each group,and the protein expressions of P-ERK,CREB,P-CREB and BDNF were detected by Western Blotting.Results:1)The general condition of group M rats had obvious CFS characteristics;2)Compared with group C,the cells in the hippocampal CA1 area of group M were not significantly damaged,while in the CA3 area and DG area were significantly damaged.In the MC group,there was cell damage in the hippocampal CA1 area,and the in the CA3 area and DG area was improved.The morphological structures of the rat cells in ME group,CC group and CE group were normal.3)Compared with group C,P-ERK protein expression was significantly decreased in the hippocampus of group M(P<0.05),and the expression of CREB and BDNF protein was significantly decreased(P<0.01).There was no significant difference in the expression of P-CREB protein(P>0.05).4)Compared with the CC group,the expression levels of P-ERK,CREB,P-CREB and BDNF in the hippocampal tissues of the CE group were significantly increased(P<0.01).The expressions of P-ERK and CREB proteins in the hippocampal tissues of the MC group were significantly increased(P<0.01),and the expressions of P-CREB and BDNF proteins were significantly decreased(P<0.01).Compared with the CE group,the expression of P-ERK protein in the hippocampus of the ME group was significantly higher(P<0.01),and the expression of CREB,P-CREB and BDNF protein was significantly lower(P<0.01).Conclusion:The damage of hippocampal neurons induced by chronic fatigue syndrome may be directly related to ERK/CREB/BDNF signaling pathway.Aerobic exercise can promote the expression of ERK/CREB/BDNF signaling pathway protein in the hippocampus of rats and thus repair the damage of hippocampal neurons.
作者
李艳荣
LI Yanrong(School of Physical Education, Chaohu University, Hefei 238000, Anhui, China)
出处
《山东体育学院学报》
北大核心
2020年第2期97-105,共9页
Journal of Shandong Sport University
关键词
慢性疲劳综合征
有氧运动
海马
细胞外调节蛋白激酶
环磷酸腺苷反应元件结合蛋白
脑源性神经营养因子
神经元可塑性
chronic fatigue syndrome
aerobic exercise
hippocampal
extracellular regulated protein kinases(ERK)
cyclic adenosine monophosphate response element binding protein(CREB)
brain-derived neurotrophic factor(BDNF)
neuronal plasticity
