摘要
研究同时提取虎杖中虎杖苷和白藜芦醇的最佳工艺.采用正交设计法优选提取分离条件,借助HPLC法测定虎杖苷和白藜芦醇的含量.以乙醇为提取溶剂,超声波辅助提取,最优条件为:80%乙醇,料液比为1∶20,提取温度为60℃,提取时间为40 min,虎杖苷和白藜芦醇的提取含量分别为2.42%和0.41%.研究结果表明,所选工艺稳定性好,操作简单.
The optimum technology of simultaneously extracting polygonin and resveratrol from polygonum cuspidatum is studied.Orthogonal design method is used to optimize the extraction and separation conditions,and HPLC method is used to determine their contents.The ethanol as extraction solvent,ultrasonic assisted extraction,the optimum condition is 80%ethanol,and solid-liquid ratio of 120,extraction temperature of 60℃,extracting time for 40 min,giant knotweed glycosides and the extraction of resveratrol content of 2.42%and 0.41%respectively.A conclusion is conducted that the selected process is good in stability,and simple in operation.
作者
张玉千
张宇
龚赛男
许瑾
杨阳春
Zhang Yuqian;Zhang Yu;Gong Sainan;Xu Jin;Yang Yangchun(School of Chemistry and Bioengineering,Nanjing Normal University Taizhou College,Taizhou 225300,China)
出处
《南京师范大学学报（工程技术版）》
CAS
2020年第2期88-92,共5页
Journal of Nanjing Normal University(Engineering and Technology Edition)
基金
江苏省高校自然科学基金项目(16KJB180017)
2019江苏省大学生实践创新训练项目
特色植物资源天然产物创新产品研发应用项目(NY-011-2015)。
关键词
虎杖苷
白藜芦醇
提取
正交设计
polydatin
resveratrol
extraction
orthogonal design
