摘要
目的研究荜拨(piperlongumine,PL)体外抗新孢子虫的作用。方法通过细胞毒性试验确定PL对Vero细胞增殖率完全无影响的安全浓度,用安全浓度的PL处理Vero细胞,检测PL对细胞内新孢子虫增殖、细胞感染率以及虫体超微结构的影响。结果处理24和48 h,PL浓度为0.6和1.25μg/mL对Vero细胞活力均无影响;PL呈剂量依赖性抑制Vero细胞内新孢子虫增殖,非剂量依赖性降低Vero细胞的感染率,可显著损伤新孢子虫的正常形态结构,使虫体胞质水肿,大量空泡产生,类锥体与棒状体丢失,虫体崩解。结论PL能显著抑制细胞内新孢子虫的增殖,降低虫体感染Vero细胞的能力,损伤虫体的超微结构,发挥较好的抗新孢子虫作用,在治疗新孢子虫病方面具有潜在的药用价值。
Objective To investigate the effect of piperlongumine(PL)against Neospora caninum infection in vitro.Methods The safe concentration of PL which showed no effect on the vitality of Vero cells was determined by cytotoxicity test.Vero cells were treated with PL at the safe concentration,and the effects of PL on propagation of N.caninum in cells,cell infection rate and ultrastructure of tachyzoites were evaluated.Results Treatments with PL at concentrations of 0.6 and 1.25μg/mL for 24 and 48 h showed no influence on the vitality of Vero cells.PL inhibited the propagation of N.caninum in cells in a dose-dependent pattern and decreased the infection rate of Vero cells in a dose-independent pattern.Meanwhile,PL significantly damaged the normal morphology of N.caninum,causing the swelling of parasites,formation of a large quantity of vacuoles,losses of conoids and rhoptries and disintegration of parasites.Conclusion PL significantly inhibited the propagation and infection ability of N.caninum and damaged the ultrastructure of the parasite,and showed good effect against N.caninum infection and a potential medicinal value in the treatment of neosporosis.
作者
李璐
王晓岑
张楠
宫鹏涛
李建华
李新
马丹
关贵全
张西臣
LI Lu;WANG Xiao-cen;ZHANG Nan;GONG Peng-tao;LI Jian-hua;LI Xin;MA Dan;GUAN Gui-quan;ZHANG Xi-chen(Key Laboratory of Zoonosis Research,Ministry of Education,College of Veterinary Medicine,Jilin University,Changchun 130062,Jilin Province,China)
出处
《中国生物制品学杂志》
CAS
CSCD
2020年第5期516-520,共5页
Chinese Journal of Biologicals
基金
国家自然科学基金项目(31902296)
国家重点基础研究发展计划(2015CB150300)
家畜疫病病原生物学国家重点实验室(中国农业科学院兰州兽医研究所)开放课题(SKLVEB2019KFKT006)。
关键词
新孢子虫
荜拨
VERO细胞
增殖
细胞感染率
超微结构
Neospora caninum
Piperlongumine(PL)
Vero cells
Propagation
Cell infection rate
Ultrastructure
