摘要
通过检测重金属离子(Cu^2+,Zn^2+,Pb^2+,Hg^2+,Cd^2+)处理后‘广林9号桉’(’Eucalyptus Guanglin9’)4个叶绿体基因(Rbcl,Accd,Ndhf,Matk)的表达变化,探究重金属胁迫对叶绿体基因表达的影响。分别用5种重金属离子的低、中、高浓度溶液连续灌根处理‘广林9号桉’3月龄幼苗20 d后,提取总RNA,反转录获得cDNA,实时荧光定量PCR(qPCR)检测Rbcl、Accd、Ndhf、Matk基因的相对表达水平。Zn^2+、Pb^2+、Hg^2+处理后,基因表达总体表现为:低浓度重金属离子诱导叶绿体基因表达水平略微升高;中高浓度重金属离子则显著抑制基因表达。除低浓度Cd^2+抑制Rbcl表达外,不同浓度Cd^2+对Rbcl、Accd、Ndhf、Matk基因的表达水平均有显著促进作用。高浓度Cu^2+促进Accd和Ndhf的表达,其它Cu^2+浓度则抑制4个叶绿体基因的表达。结果表明:重金属离子对桉树叶绿体基因表达的影响与离子种类和浓度有关。中、高浓度Zn^2+、Pb^2+、Hg^2+抑制基因表达,中、高浓度Cd^2+促进基因表达。本试验结果可为桉树重金属抗性研究提供参考。
The effects of heavy metal ions(Cu^2+,Zn^2+,Pb^2+,Hg^2+ and Cd^2+)on chloroplast gene expression were studied by detecting the expressions of four chloroplast genes(Rbcl,Accd,Ndhf,Matk)in Eucalyptus.Using threemonth-old seedlings of’Eucalyptus Guanglin9’(Eucalyptus grandis×Eucalyptus urophlla)as material,the transcriptional changes of 4 chloroplast genes were analyzed by real-time fluorescence quantitative PCR(qPCR),after continuous root irrigation for 20 d with five kinds heavy metal solution at three different concentrations(namely low,medium and high concentrations).After treatment with Zn^2+,Pb^2+,Hg^2+,the gene expressions were slightly increased at low concentration,but were significantly inhibited at medium and high concentration.Cd^2+inhibited the expression of Rbcl at low concentration.However,Cd^2+at medium and high concentration signifi-cantly promoted the gene expression of Rbcl,Accd,Ndhf and Matk.Except that the expressions of Accd and Ndhf were promoted by high concentration of Cu^2+,the expressions of four chloroplast genes were inhibited by all concentrations of Cu^2+.The results indicated that the effect of heavy metal ions on chloroplast gene expression was related to the type and concentration of ions.It provided a reference for the study of heavy metal resistance in Eucalyptus.
作者
古诗婷
刘梦霜
杨轶凤
吴晓媚
黄真池
Gu Shiting;Liu Mengshuang;Yang Yifeng;Wu Xiaomei;Huang Zhenchi(School of Life Science and Technology,Lingnan Normal College,Zhanjiang,524048;Engincering Center of Resource Plant,Lingnan Normal Col-lege,Zhanjiang,524048)
出处
《分子植物育种》
CAS
CSCD
北大核心
2020年第11期3549-3554,共6页
Molecular Plant Breeding
基金
国家自然科学基金(31570660)
广东省自然科学基金(2016A030313668)
大学生创新创业项目共同资助。
