摘要
近年来,具有农业、能源和环保价值的昆虫微生物种类和基因得到了开发,昆虫肠道微生物展示了其巨大的应用潜力,本研究旨在从蟋蟀后肠分离和鉴定纤维素降解细菌。首先采用羧甲基纤维素钠液体培养基对蟋蟀后肠中的微生物进行富集培养,然后使用羧甲基纤维素钠固体培养基分离和筛选单菌落,再通过16S rRNA测序对纤维素降解细菌进行分子鉴定,最后通过刚果红染色来进一步分析细菌降解纤维素的能力。从蟋蟀后肠中共分离出20株纤维素降解细菌,16S rRNA基因测序结果显示来自肠杆菌属(Enterobacter)9株,不动杆菌属(Acinetobacter)7株,克雷伯氏菌属(Klebsiella)2株,鞘氨醇杆菌属(Sphingobacterium)1株和葡萄球菌属(Staphylococcus)1株。刚果红染色试验结果显示,克雷伯氏菌属两株PDSCDXS2B和8B,鞘氨醇杆菌属PDSCDXS7C和不动杆菌属PDSCDXS12C具有较高的纤维素降解能力。这是首次从蟋蟀后肠分离和筛选出来具有纤维素降解能力的细菌,为昆虫源纤维素降解细菌的研究提供了微生物资源。
In recent years,insect microbial species and genes with agricultural,energy and environmental values have been developed,and insect intestinal microorganisms have demonstrated their great potential for application. Our purpose is to isolate and identify cellulose-degrading bacteria from the posterior intestine of crickets. First,carboxymethyl cellulose sodium liquid medium was used to enrich and cultivate microorganisms in the posterior intestine,then carboxymethyl cellulose sodium solid medium was used to separate and screen monophyletic colonies,and the cellulose degradation bacteria were identified by 16 S rRNA sequencing. Finally,the ability of bacteria to degrade cellulose was further analyzed by congolese red staining. In our research,20 cellulose-degrading bacteria were isolated from the posterior intestine of crickets. The sequencing results of the 16 S rRNA gene showed that 9 strains were from Enterobacter,7 from Acinetobacter,2 from Keleiboshi,1 from Sphingobacterium and 1 from Staphyloccus. The results of the congo red stain test showed that Keleiboshi has two PDSCDXS2 B and 8 B strains,Schwannosin is PDSCDXS7 C,and Acetobacter is PDSCDXS12 C have high cellulose degradation ability. For the first time,we isolated and screened cellulose-degrading bacteria from the posterior intestine of crickets,which provided clues and scientific basis for the study of insect-derived cellulose degradable bacteria.
作者
张科
苏智鹏
许阳
李清利
王瑜
刘丽
李冰洁
周云霞
夏西超
ZHANG Ke;SU Zhipeng;XU Yang;LI Qingli;WANG Yu;LIU Li;LI Bingjie;ZHOU Yunxia;XIA Xichao(College of Medicine,Pingdingshan University,Pingdingshan,Henan 467000,China;Animal Husbandry Bureau of Ruzhou,Ruzhou,Henan 467599,China)
出处
《生物资源》
CAS
2020年第2期228-233,共6页
Biotic Resources
基金
平顶山学院博士启动基金项目(PXY-BSQD-2015006)
河南省高等学校科学技术研究重点项目(16B180004)。
关键词
蟋蟀
纤维素降解细菌
16S
rRNA
posterior intestine
cellulose⁃degrading bacteria
16S rRNA
