摘要
权重基因共表达网络分析(Weighted Gene Co-expression Network Analysis,WGCNA)是系统生物学的一种研究方法,在多样本转录组数据中挖掘与目标性状相关的基因模块有较广泛的应用。为了深入探究马铃薯应对干旱胁迫的分子机制,本研究以国际马铃薯中心引进栽培种C16(CIP 397077.16)和C119(CIP 398098.119)为试验材料,将其无菌组培苗利用甘露醇模拟干旱胁迫,处理0 h、2 h、6 h、12 h和24 h,取其根系进行转录组测序,每样设3个生物学重复,共30个样本。基于以上转录组数据,利用WGCNA构建与抗逆生理性状相关联的权重基因共表达网络,得到15个与根系抗旱密切相关的基因共表达模块,并从4个与目标性状关联度最高的模块中发掘到数个核心基因,功能注释表明其中大部分参与干旱胁迫调控通路。这些结果为进一步研究马铃薯根系抗旱的分子遗传机制提供了线索。
Weighted gene co-expression network analysis(WGCNA)is a research method in systematic biology.It is widely used to identify gene modules related to target traits in multi-sample transcriptome data.In order to further explore the molecular mechanism of potato response to drought stress,two cultivars(C16:CIP 397077.16 and C119:CIP 398098.119)introduced from the International Potato Center were used as experimental materials,and five drought stress gradients were treated for 0 h,2 h,6 h,12 h,and 24 h,with the untreated material as a control.A total of 30 samples of root system were used for transcriptome sequencing,with three biological replicates.Based on the above transcriptome data,we constructed a co-expression network of weighted genes associated with stress-resistant physiological traits by WGCNA,and obtained 15 gene co-expression modules closely related to root drought resistance.In addition,a number of hub genes involved in drought stress regulation pathways were discovered from the four modules with the highest correlation with target traits.These results provide clues for further study on the m olecular genetic mechanisms of potato root drought resistance.
作者
秦天元
孙超
毕真真
梁文君
李鹏程
张俊莲
白江平
QIN Tian-Yuan;SUN Chao;BI Zhen-Zhen;LIANG Wen-Jun;LI Peng-Cheng;ZHANG Jun-Lian;BAI Jiang-Ping(Gansu Provincial Key Laboratory of Aridland Crop Science/Gansu Key Laboratory of Crop Improvement&Germplasm Enhancement,Lanzhou 730070,Gansu,China;College of Agronomy,Gansu Agricultural University,Lanzhou 730070,Gansu,China)
出处
《作物学报》
CAS
CSCD
北大核心
2020年第7期1033-1051,共19页
Acta Agronomica Sinica
基金
国家自然科学基金项目(31660432,31460369)
国家现代农业产业技术体系(马铃薯)建设专项(CARS-09-P14)
甘肃省马铃薯产业体系(GARS-03-P1)
中国科学院“西部之光”人才培养计划(2014-01)
兰州市科技发展计划(2015-3-62)
甘肃省教育厅项目(2019B-073)
甘肃省自然科学基金(18JR3RA174)
甘肃农业大学国重实验室开放基金(GSCS-2017-9)
甘肃农业大学创新基金(GAU-XKJS-2018-085)项目资助。
