摘要
通过原子转移自由基聚合(ATRP)方法制备了以多面体低聚倍半硅氧烷(POSS)为骨架的POSS-(PDMAEMA)8(PPD)星型阳离子聚合物,通过巯基-双键化学反应,将特异性靶向EA.hy926内皮细胞的CREDVW多肽键接在PPD阳离子聚合物末端,得到POSS-(PDMAEMA)8-PPEGMA-CREDVW(PPD-CREDVW)阳离子聚合物.在n(N)/n(P)=5时,PPD-CREDVW阳离子聚合物携载pEGFP-ZNF580(pDNA)质粒自组装形成粒径为125.67±3.31 nm、zeta电位为10.64±1.65 mV的PPD-CREDVW/pDNA基因复合胶束.细胞实验结果显示:与PPD/pDNA基因复合胶束相比,PPD-CREDVW/pDNA基因复合胶束携载基因能力强、细胞毒性低、易被细胞摄取,能够显著提高EA.hy926内皮细胞的转染,促进细胞的增殖.
A star-shaped cationic polymer POSS-(PDMAEMA)8(PPD)based on oligomeric sesquioxane(POSS)was prepared with atom transfer radical polymerization(ATRP).In order to prepare POSS-(PDMAEMA)8-PPEGMA-CREDVW(PPD-CREDVW)cationic polymer,the CREDVW peptide,which targets endothelial cells(EA.hy926),was grafted onto the end of PPD cationic polymer through the thiol-double bond click chemical reaction.When n(N)/n(P)=5,PPD-CREDVW cationic polymer loaded with pEGFP-ZNF580(pDNA)plasmid could form PPD-CREDVW/pDNA gene composite micelle with a particle size of 125.6±3.31 nm and a zeta potential of 10.64±1.65 mV through self-assembly.Compared with PPD/pDNA gene complexes micelles,PPD-CREDVW/pDNA gene complexes micelles could be uptaken by cells,had strong capacity for gene-carrying and had low cytotoxicity.Thus it can significantly promote the transfection and proliferation of endothelial cells.
作者
朵兴红
姜萧韩
周若琦
冯亚凯
刘晶
DUO Xinghong;JIANG Xiaohan;ZHOU Ruoqi;FENG Yakai;LIU Jing(School of Chemistry and Chemical Engineering, Qinghai University for Nationalities, Xining 810007, China;School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;College of Eco-environment and Resources, Qinghai University for Nationalities, Xining 810007, China;Key Laboratory of Resource Chemistry and Eco-environmental Protection in Tibetan Plateau, State Ethnic Affairs Commission, Xining 810007, China)
出处
《华南师范大学学报（自然科学版）》
CAS
北大核心
2020年第3期62-69,共8页
Journal of South China Normal University(Natural Science Edition)
基金
国家自然科学基金项目(51963018)
青海省自然科学基金项目(2019-ZJ-7013)
教育部“春晖计划”合作研究项目(Z2015049)
青海民族大学高层次人才项目(2019XJG01)
青海民族大学2020年度校级本硕博(学生)项目(2020XJXS12)。
