摘要
【目的】分析不同立地条件沙田柚果园土壤微生物多样性,为沙田柚的精准施肥和精细管理提供参考依据。【方法】以广西容县沙田柚果园五一柚场为研究对象,测定该区域不同立地条件(坡向和坡位)土壤的基础理化性质(总氮、水分含量和pH),同时利用高通量测序技术对其微生物群落结构及多样性进行分析。【结果】土壤样本共获得37806个操作分类单元(OTUs),至少涵盖46门57纲136目270科661属的细菌;门水平聚类分析结果表明,沙田柚种植土壤与对照(未种植沙田柚的空地)间能明显区分开,变形菌门(Proteobacteria)和拟杆菌门(Bacteroidetes)是沙田柚种植土壤的优势菌门;Chujaibacter是沙田柚种植土壤微生物的优势属,但其相对丰度在不同坡向和坡位差异较明显,北坡向(25.77%)和下坡位(16.86%)相对丰度最高,而东坡向(5.26%)和上坡位(5.58%)较低;Pseudomonas cichorii是沙田柚种植潜在的致病种。土壤理化性质分析发现,坡位仅对土壤水分含量产生显著影响(P<0.05,下同),下坡位水分含量最高(58.25%),上坡位最低(47.00%),二者间差异显著;坡向是影响沙田柚种植土壤总氮、水分和pH的主要因素,其中,西坡向土壤水分含量最高(63.00%)、南坡向最低(42.67%),南坡向pH最高(6.60)、东坡向最低(5.90),北坡向土壤的总氮含量最高(2.07 g/kg)、南坡向最低(1.77 g/kg)。土壤水分含量影响土壤微生物4个属和5个种,总氮含量影响2个属和2个种,而p H对土壤微生物结构未产生显著影响(P>0.05);另有3个属和2个种的微生物可能与沙田柚品质相关。【结论】坡向对沙田柚种植土壤的理化性质影响较大,沙田柚种植影响土壤细菌群落组成,坡向和坡位造成沙田柚果园土壤细菌群落组成差异。
【Objective】The soil microbial diversity of Shatian pomelo orchard was analyzed under different site conditions to provide reference for precise fertilization and disease control of Shatian pomelo.【Method】Taking the Wuyi pomelo orchard in Rongxian County of Guangxi as the object,the basic physical and chemical properties(total nitrogen,water content and pH)of soil under different site conditions(slope direction and position)were analyzed.Meanwhile,the microbial community structure and diversity analysis were carried out by high-throughput sequencing technology.【Result】The results showed that the total number of operational taxonomic unit(OTUs)obtained in this study was 37806,including least 46 phyla,57 classes,136 orders,270 families and 661 genera.The cluster analysis at phyla level indicated that the soil cultivating Shatian pomelo orchard and control(with no Shatian pomelo)could be separated easily.Proteobacteria and Bacteroidetes were dominant bacteria populations at phylum level.Chujaibacter was the dominant genus of soil microbe in Shatian pomelo orchard,but its relative abundance varied greatly in different slope directions and positions,the relative abundances of north slope direction(25.77%)and lower slope(16.86%)were higher,those of east slope direction(5.26%)and upper slope(5.58%)were lower.Pseudomonas cichorii was a potential pathogen in Shatian pomelo cultivation.Analysis of soil physical and chemical properties showed that slope position significantly affected water content(P<0.05,the same below),the highest water content(58.25%)was in the lower slope,the lowest(47.00%)was in the upper slope,and the difference between the two was significant.The slope direction affected soil total nitrogen,water content and pH,among which the soil water content in the north slope was the highest(63.00%),the south slope was the lowest(42.67%),the pH value in the south slope was the highest(6.60),the east slope was the lowest(5.90),the total nitrogen content in the north slope was the highest(2.07 g/kg),and the south slope was the lowest(1.77 g/kg).Soil water content affected 4 genera and 5 species of soil bacteria,total nitrogen content affected 2 genera and 2 species,pH had no significant effects on soil microbial community composition(P>0.05);and 3 genera and 2 species of soil bacteria might be related to the quality of Shatian pomelo.【Conclusion】This study demonstrates that the slope direction factor has great influence on the physical and chemical properties of Shatian pomelo cultivating soil.The cultivation of Shatian pomelo affects the soil microbial community composition.The slope direction and position contribute a lot to the differences of soil microbial community composition in Shatian pomelo orchard.
作者
彭玉娇
崔学宇
谭梦超
阮红燕
邵元元
曾文萍
区燕丽
侯彦林
PENG Yu-jiao;CUI Xue-yu;TAN Meng-chao;RUAN Hong-yan;SHAO Yuan-yuan;ZENGWen-ping;OU Yan-li;HOU Yan-lin(Guangxi Geographical Indication Crops Research Center of Big Data Mining and Experimental Engineering Technology/Key Laboratory of Beibu Gulf Environment Change and Resources Utilization of Ministry of Education/Guangxi Key Laboratory of Earth Surface Processes and Intelligent Simulation,Nanning Normal University,Nanning 530001,China;Soil and Fertilizer,Plant Protection and Cash Crops Station of Rongxian,Rongxian,Guangxi 537500,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2020年第5期1136-1144,共9页
Journal of Southern Agriculture
基金
广西科技重大专项(桂科AA17204077)
广西一流学科(地理学)建设项目(2019)
北部湾环境演变与资源利用教育部重点实验室系统基金项目(GTEU-KLOP-X1820,GTEU-KLOP-X1819)。
关键词
沙田柚
立地条件
微生物群落结构
高通量测序技术
Citrus maxima(Burm.)Merr.cv.Shatian Yu
site condition
microbial community structure
high throughput sequen-cing technology
