摘要
目的分析3种不同固定相对皂苷异构体的分离效果。方法3对皂苷异构体gypenoside L和gypenoside LI、damulin A和damulin B、20(S)-ginsenoside Rg3和20(R)-ginsenoside Rg3,通过C18色谱柱、五氟苯基丙基色谱柱及双苯基色谱柱进行分离比较。以Inertsill ODS-SP色谱柱、Shim-pack Velox PFPP色谱柱和Shim-pack Velox Bipheny1色谱柱作为固定相,用不同比例的流动相分离gypenoside L、gypenoside LI、damulin A、damulin B、20(S)-ginsenoside Rg3和20(R)-ginsenoside Rg3。结果Shim-pack Velox PFPP色谱柱分别在29%和33%乙腈水溶液以1.0 mL/min等度洗脱,gypenoside L和gypenoside LI的分离度达到2.27,20(S)-ginsenoside Rg3和20(R)-ginsenoside Rg3的分离度达到3.50,分离效果最佳;Shim-pack Velox Biphenyl色谱柱在33%乙腈水溶液以0.5 mL/min等度洗脱,damulinA和damulinB的分离效果最佳,分离度达到2.73。结论五氟苯基丙基色谱柱因键合五氟苯基丙基,对差向异构体具有更好的分离度,双苯基色谱柱因键合二苯基,对位置异构体有更好的分离度。
Objective To analyze the separation effects of 3 different stationary phases on saponin isomer.Methods Three pairs of saponin isomers,gypenoside L and gypenoside LI,damulin A and damulin B,20(S)-ginsenoside Rg3 and 20(R)-ginsenoside Rg3,were compared by C18 column,pentafluorophenylpropyl(PFPP)column and diphenyl column,respectively.Inertsill ODS-SP column,Shim-pack Velox PFPP column and Shim-pack Velox Diphenyl column were used as stationary phases,and gypenoside L,gypenoside LI,damulin A,damulin B,20(S)-ginsenoside Rg3 and 20(R)-ginsenoside Rg3 were separated by different proportions of mobile phase.Results The effects of separation of gypenoside L and gypenoside LI,and 20(S)-ginsenoside Rg3 and 20(R)-ginsenoside Rg3 showed the best using Shim-pack Velox PFPP column.The resolution of gypenoside L and gypenoside LI was 2.27 eluted with 29%acetonitrile and the resolution of 20(S)-ginsenoside Rg3 and 20(R)-ginsenoside Rg3 was 3.50 eluted with 33%acetonitrile at flow rate of 1.0 mL/min;Damulin A and damulin B showed the best separation,with the resolution of 2.73,using Shim-pack Velox Diphenyl column eluted with 33%acetonitrile at the flow rate of0.5 mL/min.Conclusion Pentafluorophenylpropyl column is used to separate the epimers due to the bonding of pentafluorophenylpropyl.Diphenyl column shows better resolution for positional isomers due to the bonding of diphenyl groups.
作者
李心璨
唐天昕
余辰茜
杨飞雪
朴香兰
LI Xin-Can;TANG Tian-Xin;YU Chen-Qian;YANG Fei-Xue;PIAO Xiang-Lan(School of Pharmacy,Minzu University of China,Beijing 100081,China)
出处
《食品安全质量检测学报》
CAS
2020年第5期1502-1507,共6页
Journal of Food Safety and Quality
基金
国家自然科学基金项目(81673692)
国家大学生创新训练计划项目(GCCX2019110036)。
