摘要
综述了百合分子生物学的研究进展,主要包括4个方面,即百合基因组学、转录组学和蛋白组学的研究;百合遗传多样性分析和5种分子标记(RAPD、ISSR、SSR、AFLP、SRAP)的开发和利用;百合遗传转化体系的构建,包括农杆菌介导法、基因枪法、电激法和花粉管介导法;百合减数分裂基因、花发育基因及抗逆性相关基因的克隆和表达分析。最后指出了当前研究存在的问题,并展望了进一步研究的方向。
This paper reviewed the following four aspects in lily molecular biology:research progresses of lily genomics,transcriptomics and proteomics;the development and utilization of five types of molecular markers including RAPD,ISSR,SSR,AFLP and SRAP in lily genetic diversity analysis;construction of lily transformation system including Agrobacterium mediated method,gene gun bombardment method,electroporation method and pollen tube pathway method;cloning and expression analysis of genes related to meiosis,flower development and stress resistance.Finally,the current research defects and prospects of lily molecular biology were summarized.
作者
方洁
潘俊杰
程科军
吕群丹
FANG Jie;PAN Jun-jie;CHENG Ke-jun;LV Qun-dan(Lishui Institute of Agriculture and Forestry Sciences,Lishui Zhejiang 323000)
出处
《现代农业科技》
2020年第12期69-73,76,共6页
Modern Agricultural Science and Technology
基金
丽水市科技项目(2017ZDYF07,LS20160012)。
关键词
百合
分子标记
遗传转化
基因克隆
lily
molecular marker
genetic transfomation
gene cloning
