摘要
以宁夏优良品种欧亚种酿酒葡萄(Vitis viniferalL.)赤霞珠(Cabernet sauvignon)为试材,通过大田叶面喷施不同浓度亚硒酸钠,监测发育期果实总糖、还原糖、可溶性固形物、可滴定酸、pH值、总酚、总黄烷醇和花色苷含量以及矿质元素含量,并测定果实DPPH自由基清除活性、ABTS自由基阳离子清除活性和总还原力,分析外源硒对酿酒葡萄果实品质的影响。结果表明,随着果实成熟期的推进,硒对浆果中Se、总糖、还原糖、可溶性固形物、P、Cu、Mn和Mg元素的含量及其总还原力、DPPH自由基清除活性和ABTS自由基清除活性具有显著的正效应,对可滴定酸和果籽、果皮及果肉酚类物质含量的影响因浓度不同而异,但对pH值、K、Zn、Fe、Ca、N-NH4、N-NO3和S元素含量无显著性影响。不同处理间相比较,Se10处理中果实总酚和Mn元素含量最高,Se25处理中果实总黄烷醇含量最高,Se50处理中果实还原糖、可滴定酸和花色苷含量及总还原力、ABTS自由基清除活性最高,Se75处理中果实Se、总糖、可溶性固形物、P、Cu和Mg元素含量及DPPH自由基清除活性最高。采用灰色系统关联度理论,选取不同处理间具有显著差异的理化指标进行灰色关联度分析,Se50处理的酿酒葡萄果实理化特性加权关联度最高,故叶面喷施50 mg/L亚硒酸钠的果实质量最佳。
Cabernet sauvignon wine grape(Vitis viniferal L.)were selected as the research material.By spraying sodium selenite in the field,the influences of different concentrations of selenium on wine grape fruit quality were investigated through monitoring total sugar,reducing sugar,soluble solids,titratable acid,pH,total phenol,total flavanol,anthocyanin content and mineral element content of the fruit during development period,as well as determining the DPPH free radical scavenging activity and ABTS free radical cation scavenging activity and total reducing power of wine grapes.The results showed that selenium had a significant positive effect on the contents of total sugar,reducing sugar,soluble solids,P,Cu,Mn,and Mg elements,and the total reducing power,DPPH radical scavenging activity and ABTS radical scavenging activity in berries with the process of fruit maturity,but had no significant effect on pH value,K,Zn,Fe,Ca,N-NH 3,N-NO 4 and S elements in fruits.The influence of selenium on titratable acid content and phenolic substances content in seeds,skins and flesh varied with different selenium concentrations.The contents of total phenols and Mn were the highest in Se10 treatment,the content of total flavanols was the highest in Se25 treatment,the contents of reducing sugar,titratable acid and anthocyanin and the reducing ability and ABTS free radical scavenging activity were the highest in Se50 treatment,and the contents of total sugar,soluble solids,P,Cu,and Mg and the scavenging activity of DPPH free radical were the highest in Se75 treatment.The grey system relational degree was adopted to make comprehensive evaluation of fruit quality.The indicators with significant differences among different treatments were selected for grey relational degree analysis.The weighted relational degree of physicochemical characteristic in wine grapes was the highest in Se50 treatment.Thus,the fruit quality is the best when 50 mg/L sodium selenite was sprayed on the leaves.
作者
朱娟娟
马海军
李敏
杜梦
田婷婷
ZHU Juanjuan;MA Haijun;LI Min;DU Meng;TIAN Tingting(College of Biological Science and Engineering,North Minzu University,Yinchuan 750021,China;Ningxia Grape&Wine Innovation Center,Yinchuan 750021,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2020年第12期127-133,共7页
Food and Fermentation Industries
基金
中央高校基本科研业务费专项(2017kj30)。