摘要
作为世界第四大粮食作物,马铃薯(Solanum tuberosum)经常受到盐碱等非生物胁迫从而造成产量减少。作为重要的转录因子之一, CBFs (C-repeat binding factor)在植物抵抗低温方面起到了重要作用,但对于其耐盐能力的研究还很少。为研究马铃薯中StCBFs是否能提高植物耐盐性,我们选择转StCBF1基因过表达拟南芥(Arabidopsis thaliana)株系StCBF1-2和StCBF1-5与转StCBF4基因过表达拟南芥株系StCBF4-2和StCBF4-3作为实验材料进行相关生理生化指标的测定。结果表明,转基因拟南芥能提高盐胁迫下拟南芥中四种主要抗氧化酶超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性,降低超氧阴离子含量以缓解植物体内的氧化胁迫。同时通过荧光定量PCR分析发现,转基因拟南芥的COR基因以及Na^+/H^+逆向转运蛋白相关基因的表达量要高于野生型拟南芥,这也可能是其耐盐性强的原因之一。
As the fourth-largest food crop in the world, potatoes are often subjected to abiotic stresses such as salt stress, resulting in reduced yields. As one of the important transcription factors, CBFs play an important role in plant resistance to low temperature, but little research has been done on their role in salt tolerance. We use StCBF1 transgenic lines and StCBF4 transgenic lines of Arabidopsis as material to explore the physiological and molecular mechanism of StCBFs in improving salt tolerance of plants. The results showed that, under salt stress, both StCBF1 and StCBF4 transgenic lines increase the activities of SOD, POD, CAT and APX in Arabidopsis decrease the content of superoxide anion and H2 O2, so as to alleviate the oxidative stress in transgenic plants. At the same time, it was found that the expression of COR genes and Na^+/H^+ antiporter-related genes in transgenic Arabidopsis was higher than that in wild-type Arabidopsis, which may be associated with its high salt tolerance.
作者
宋奇娉
刘洋
CHEN Tony Huihuang
杨兴洪
SONG Qiping;LIU Yang;CHEN Tony Huihuang;YANG Xinghong(State Key Laboratory of Crop Biology,College of Life Sciences,Shandong Agricultural University,Taian,Shandong 271018,China;Department of Horticulture,Oregon State University,Corvallis,Oregon 97331,USA)
出处
《植物生理学报》
CAS
CSCD
北大核心
2020年第3期389-398,共10页
Plant Physiology Journal
关键词
马铃薯
拟南芥
StCBFs
耐盐性
Solanum tuberosum
Arabidopsis thaliana
StCBFs
salt tolerance
