摘要
元宝枫是我国特有树种,其籽含有丰富的不饱和脂肪酸和多种营养物质。本研究旨在探究元宝枫籽对小鼠肠道菌群组成结构的影响以及对有害菌的抑制作用。选取8周龄的昆明小鼠,元宝枫籽饲喂添加量为1.5 g/d,连续饲喂2周后采集直肠粪便进行肠道菌群的高通量测序分析,并且全程记录体质量变化。通过对小鼠肠道菌群分析后发现:实验组与对照组小鼠肠道菌群的α多样性并未表现出显著差异;通过β多样性分析发现实验组与对照组数据发生显著性分离(P<0.05)。通过对组间分类学水平数据对比发现:相比于对照组,实验组中厚壁杆菌门的相对丰度增高而拟杆菌门的相对丰度下降;比较组间厚壁杆菌门和拟杆菌门相对丰度比值的差异变化,发现实验组小鼠中的比值高于对照组(P=0.063)。通过线性判别分析效应量发现:相比于对照组,实验组中Staphylococcus和Acinetobacter的丰度要显著低于对照组,表明元宝枫籽对机会性致病菌有一定的抑菌作用。结果表明,元宝枫籽可以影响小鼠肠道菌群的结构组成,同时对机会性致病菌有一定的抑菌作用。
Acer truncatum is a tree species unique to China.Its seeds contain abundant unsaturated fatty acids and multiple nutritional components.The aim of this study was to explore the effects of Acer truncatum seeds on the composition of the intestinal flora in mice and the inhibition of harmful bacteria.Eight-week-old Kunming mice were selected and fed 1.5 g of Acer truncatum seeds every day.After continuous feeding for two weeks,rectal feces were collected for high-throughput sequencing analysis and body mass recorded every day over the entire period.Theα-diversity of the intestinal flora did not significantly differ between the experimental and control groups.In theβ-diversity analysis,significant separation was found between the two groups(P<0.05).Compared with the control group,the relative abundance of Firmicutes in the experimental group was increased whereas the relative abundance of Bacteroidetes was decreased.The relative abundance ratio between Firmicutes and Bacteroides was higher in the experimental group than that in the control group(P=0.063).By linear discriminant analysis effect size analysis,it was found that the relative abundance of Staphylococcus and Acinetobacter was significantly lower in the experimental group,indicating that Acer truncatum seeds can inhibit the proliferation of harmful bacteria.In summary,Acer truncatum seeds can affect the structure and composition of the mouse intestinal flora and inhibit opportunistic pathogens.
作者
孙朋浩
薛玉环
吴永继
任玮
郑伟
朱晓岩
赵善廷
SUNPenghao;XUEYuhuan;WU Yongji;REN Wei;ZHENG Wei;ZHU Xiaoyan;ZHAOShanting(Neurobiology Laboratory,College of Veterinary Medicine,Northwest A&F University,Yangling 712100,China;Key Laboratory of Plant Nutrition and Agri-environment of Northwest,Ministry of Agriculture and Rural Affairs,College of Natural Resources and Environment,Northwest A&F University,Yangling 712100,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2020年第11期184-193,共10页
Food Science
基金
陕西省重点研发计划项目(2018ZDXM2)
西北农林科技大学2019基本科研业务费前沿与交叉科学研究项目(2452019200)。
关键词
元宝枫籽
肠道菌群
Α多样性
Β多样性
线性判别分析
Acer truncatum seed
intestinal flora
α-diversity
β-diversity
linear discriminant analysis
