摘要
研究通过体外酶解试验,评估不同蛋白酶(酸性蛋白酶、中性蛋白酶和碱性蛋白酶)、不同酶解参数(加酶量、酶解时间、含水量等)对大豆皮抗原蛋白降解效果的影响。结果表明:碱性蛋白酶对球蛋白和β-伴大豆球蛋白降解效果优于其他酶;大豆皮中添加50、75 U/g和100 U/g碱性蛋白酶对球蛋白和β-伴大豆球蛋白降解效果优于其他酶添加量,且三者效果差异不大;酶解48 h和72 h时其对大豆皮抗原蛋白的降解效果优于酶解24 h,且二者效果差异不明显;大豆皮酶解体系含水量为45%时对抗原蛋白的降解效果优于含水量为40%和50%的酶解体系。综上所述,碱性蛋白酶在降解大豆皮中球蛋白和β-伴大豆球蛋白方面具有重要的作用。其酶解参数为含水量45%、酶解时间48 h、加酶量50 U/g时,其对大豆皮中抗原蛋白降解效果相对较好,其抗原蛋白降解率达80%以上。
This study was conducted to explore the effects of different proteases(acidic protease,neu⁃tral protease and alkalic protease)and process parameters on degradation effect of antigen proteinin soybean skin,which the process parameters included the ratio of water,enzyme dose and treatment time and so on.The results showed that under the conditions of this experiment,alkalic protease had a better degradation effect on globulin andβ-conglycinin than other proteases.The enzyme dose for 50,75 U/g and 100 U/g alkalic protease to soybean hulls had the better degradation effect globulin andβ-conglycinin than that of other enzymes,and the difference among the three were not significant.The degradation effect of soybean hull antigen protein at 48 h and 72 h were both better than that of enzy⁃matic hydrolysis for 24 h,moreover,the difference for 48 h and 72 h is not significant.The degrada⁃tion effect of antigenic protein is better than those ratios of water with 40%and 50%when the water ratio of soybean hull enzymatic hydrolysis system is 45%.In summary,alkalic protease plays an impor⁃tant role in the degradation of soybean globulin andβ-conglycinin.The enzymatic hydrolysis parame⁃ters were 45%ratio of water,48 h treatment time and 50 U/g enzyme dosage,respectively.The degra⁃dation effect of antigen protein in soybean skin was relatively good,and the degradation rate of antigen protein was over 80%.
作者
韩丽
彭翔
严峰
王海燕
蔡辉益
张广民
Han Li;Peng Xiang;Yan Feng;Wang Haiyan;Cai Huiyi;Zhang Guangmin
出处
《饲料工业》
北大核心
2020年第12期38-41,共4页
Feed Industry
关键词
蛋白酶
抗原蛋白
酶解参数
降解率
大豆皮
proteases
antigen protein
hydrolysis parameters
degradation rate
soybean hulls
