摘要
为研究栀子中环烯醚萜类物质生物合成的分子机制,本研究以药用植物栀子果实为研究材料,利用Illumina HiSeq 4000对栀子进行转录组测序,利用Trinity软件对测序结果进行De novo组装,使用BLAST软件对Unigene进行分类、功能注释,通过MISA软件分析SSR位点。共组装获得75539条Unigene,平均长度1614 bp,在数据库的注释成功率为100%,共搜索到50571个SSR位点,发现栀子转录组中有1294个Unigene参与到16个与栀子次生代谢物质生物合成的代谢通路中,其中有77个参与环烯醚萜类物质生物合成。本研究初步获得栀子中参与环烯醚萜类物质合成的功能基因,不仅为开展栀子功能基因的鉴定和克隆提供基础序列,还为后续开展栀子药效成分生物合成的分子机制提供依据。
In order to study the molecular mechanism of the biosynthesis of iridoids in Gardenia jasminoides Ellis,this study used medicinal the fruit of G.jasminoides as the research material.The transcriptome was transcribed by Illumina HiSeq 4000 high-throughput sequencing platform.The sequencing results were De Novo assembled using Trinity software.Unigene was classified and functionally annotated using BLAST software.The SSR locus was analyzed by MISA software.A total of 75539 unigenes were obtained by co-assembly,with an average length of 1614 bp.The success rate of annotation was 100%.A total of 50571 SSR loci were found in the transcriptome,1294 unigenes were involved in 16 metabolic pathways involved in the biosynthesis of secondary metabolites,and 77 of them were involved in the biosynthesis of iridoids.This study preliminarily obtained the key enzyme genes involved in the formation of iridoids in G.jasminoides,provides basic sequences for identification and cloing of key genes in G.jasminoides,and constructs a basis for the molecular mechanism and pathway analysis of the biosynthesis of iridoids.
作者
潘媛
陈大霞
李隆云
Pan Yuan;Chen Daxia;Li Longyun(Chongqing Academy of Chinese Material Medica,Chongqing Sub-center of National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Science,Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica,Chongqing,400065)
出处
《分子植物育种》
CAS
CSCD
北大核心
2020年第12期3923-3931,共9页
Molecular Plant Breeding
基金
重庆市基本科研业务费(cstc2018jxjl-jbky130011)
重庆市基础与前沿研究计划项目(cstc2018jcyjAX0553)
国家中药材产业技术体系资助(CARS-21)
重庆市中药材产业技术体系(2018[5]号)
重点产业共性关键技术创新专项资助(cstc2016zdcy-ztzx10003)共同资助。
