摘要
侵袭性念珠菌病缺乏特异性临床症状和体征,传统的培养方法诊断敏感度低,且检验周期长。近年来,念珠菌非培养法因其快速、灵敏的特点而得到广泛应用,主要包括检测念珠菌的碳水化合物(1,3-β-D-葡聚糖、甘露聚糖及其抗体)、蛋白质、核酸(PCR、T2candida)。本文阐述了这些方法在诊断念珠菌病中的临床应用价值及局限性,旨在为临床早期、快速诊断侵袭性念珠菌病提供参考。
Invasive candidiasis(IC)is lack of specific clinical symptoms and signs.Conventional microbiological culture for IC diagnosis presents poor sensitivity and time-consuming.Non-culture-based methods include the detection of candida carbohydrates(1,3-β-D-glucan,mannan/anti-mannan),candida protein and candida nucleic(PCR,T2Candida).Because they are sensitive and less time-consumming,the method have been widely used for IC diagnosis.This article describes the clinical application and limitations of these non-culture methods in the diagnosis of candida diseases,to provide reference for the clinical use.
作者
陈盈竹
夏云
Chen Yingzhu;Xia Yun(Clinical Laboratory,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China)
出处
《中华临床感染病杂志》
CSCD
2020年第2期149-153,共5页
Chinese Journal of Clinical Infectious Diseases
关键词
念珠菌病
侵袭性
非培养法
诊断
Candidiasis
invasive
Non-culture method
Diagnosis
