摘要
本试验利用周林频谱仪（WS－302）照射玻璃化冷冻前后小鼠桑椹胚，研究对其体外发育的影响，结果表明：胚胎在10％EG溶液中处理5min后移入EFS30中处理1min的二步法冷冻组，解冻后经照射5～20min后的胚胎发育率（95％～100％）与对照组（94％）比较无显著性差异（P＞0．05）。当在EFS40中处理1min的一步法冷冻组的胚胎，照射后发育率由对照组的54％提高到97％差异极显著（P＜0.01）。改用GFS40一步法冷冻的胚胎照射后的发育率也明显高于对照组（P＜0．05）。另外，对冷冻前胚胎进行照射，其解冻后发育率（87％）也得到了显著提高（P＜0．01）。
This experiment was to study the developmental ability of mouse morulae which were irradiated by Zhoulin Bio-Frequency Spectrum Generator (WS-302 ) before or after being frozen. Mouse morulae were pretreated in 10% EG for 5 min,then pipetted into EFS40for another 1 min treatrnent and plunged into LN2(two-step) for storage. After thawing the embryos were irradiated by WS-302 for 5～20 min. The rate of development was 95%～100% in vitro culture, which was not differ from the control (94 % ) group (P>0. 05). However,mouse morulae were frozen only in EFS40 for 1 min(one-step),the rate of development was 97 % after the thawed embryos were irradiated by WS-302 and the control was 54%, the difference was greatly significant (P<0. 01).When the morulae were vitrified in GFS40, the developmental rate was signicantly higher than the control (P<0.05) after the thawed embryos were irradiated by WS-302. The developmental rate was also significantly higher than the control when mouse morulae were irradiated by WS-302 before freezing(P<0.01 ).
出处
《中国农业大学学报》
CAS
CSCD
北大核心
1999年第3期109-113,共5页
Journal of China Agricultural University
基金
周林频谱公司资助
