摘要
生物医学研究的发展对光学显微成像的性能,如空间分辨率、成像速度、多维度信息、成像质量等提出了更高的要求。光片荧光显微采用一个薄片光从侧面激发样品,在正交方向探测成像,具有快速三维层析成像和对样品光漂白和光毒性小的优点,是活体生物样品长时间显微观测的理想工具。本文介绍了光片荧光显微成像技术的基本原理及其主要特点;综述了光片荧光显微面临的主要技术问题,以及为解决这些问题而发展出的新原理、新思路和新方法;例举了光片荧光显微成像技术在细胞生物学、发育生物学和神经科学等领域的应用;讨论了该技术的发展趋势及前景。该研究旨在帮助研究者系统了解光片荧光显微成像技术的基本知识、最新研究发展趋势和潜在应用,为该领域科学研究提供参考。
With the development of biomedical research,more advanced and comprehensive optical microscopy are required to enhance the imaging performances such as the spatial resolution,the imaging speed,the multidimensional information,the imaging quality,etc.Light-sheet fluorescence microscopy(LSFM),illuminating the specimen with a thin light sheet from the side and obtaining the optical sectioned image orthogonally,provides an ideal tool for long-term observation of live biological specimens due to its unique features of fast volumetric imaging speed and low photobleaching and phototoxicity to samples.In this paper,we first give an introduction to the principle and properties of LSFM.Then we address the key issues existing in LSFM and the solutions based on new principles,ideas and techniques.Some applications of LSFM in cell biology,developmental biology and neurosciences are exampled,and the development trends and prospect of LSFM are discussed.The purpose of the paper is to help researchers systematically understand the principle,the state-of-the-art techniques,and the potential applications of LSFM,and provide some references for research in this field.
作者
于湘华
刘超
柏晨
杨延龙
彭彤
但旦
闵俊伟
姚保利
Yu Xianghua;Liu Chao;Bai Chen;Yang Yanlong;Peng Tong;Dan Dan;Min Junwei;Yao Baoli(State Key Laborutory of Trunsient Optics and Photonics,Xi'an Institute of Optics and Precision Mechanics,Chinese Academy of Sciences,Xi'an,Shaanxi 710119,China;Unicersity of Chinee Academry of Siences,Beijing 100049,China)
出处
《激光与光电子学进展》
CSCD
北大核心
2020年第10期1-15,共15页
Laser & Optoelectronics Progress
基金
国家自然科学基金(11704405,61905277,91750106,61705256,81427802)
关键词
显微
荧光显微
光片照明
三维成像
生物医学成像
microscopy
fluorescence microscopy
light-sheet illumination
Three-dimensional imaging
biomedical imaging
