摘要
目的探讨玉屏风水煎剂及其组分对大鼠肾脏组织及HEK-293细胞OAT1/3表达的影响。方法采用MTT法检测玉屏风及其组分水煎剂对HEK-293细胞存活率的影响;采用Western blot法和RT-PCR法检测各组水煎剂对大鼠肾脏及HEK-293细胞中OAT1/3蛋白及mRNA表达的影响。结果玉屏风、白术、防风水煎剂下调大鼠肾脏和HEK-293细胞中OAT1蛋白及mRNA表达(P<0.05,P<0.01),玉屏风及防风水煎剂下调大鼠肾脏及HEK-293细胞中OAT3蛋白及mRNA表达(P<0.05,P<0.01),白术水煎剂上调大鼠肾脏及HEK-293细胞OAT3蛋白及mRNA表达(P<0.05,P<0.01)。黄芪水煎剂对大鼠肾脏及HEK-293细胞中OAT1/3蛋白表达无抑制作用。结论玉屏风水煎剂对OAT1/3有明显的抑制作用,玉屏风的这种抑制作用可能跟其主要成分白术、防风有关。
AIM To investigate the effects of Yupingfeng Decoction(YPFD) and its components on rat kidney tissue and OAT1/3 expression in HEK-293 cells.METHODS The MTT method was used to detect the effects of YPFD and its component on the survival rate of HEK-293 cells, Western blot and RT-PCR were used to detect the effects of decoction on the expression of OAT1/3 protein and mRNA in rat kidney and HEK-293 cells.RESULTS YPFD, and decoctions of Baizhu(Atractylodis Rhizoma) and Fangfeng(Saposhnikoviae Radix) down-regulated the expression of OAT1 protein and mRNA in rat kidney and HEK-293 cells(P<0.05, P<0.01). YPFD, and Fangfeng Decoction down-regulated the expression of OAT3 protein and mRNA in rat kidney and HEK-293 cells(P<0.05, P<0.01), Baizhu Decoction up-regulated the expression of OAT3 protein and mRNA in rat kidney and HEK-293 cells(P<0.05, P<0.01). Huangqi(Astragali Radix) Decoction had no inhibitory effect on OAT1/3 protein expression in rat kidney and HEK-293 cells.CONCLUSION YPFD’s obvious inhibition on OAT1/3 may associate with its main components of Baizhu and Fangfeng.
作者
闻泽强
李大朗
宋珏
WEN Ze-qiang;LI Da-lang;SONG Jue(College of Pharmacy,Anhui Medical University,Hefei 230032,China)
出处
《中成药》
CAS
CSCD
北大核心
2020年第6期1457-1462,共6页
Chinese Traditional Patent Medicine
基金
国家自然科学基金(81803868)
安徽高校省级自然科学研究项目(KJ2014A109)
安徽省自然科学基金(1308085QH153)。
