摘要
目的为建立兽药复方磺胺嘧啶钠注射液含量测定的高效液相色谱测定法。方法流动相为0.1%的磷酸溶液-乙腈(85:15,V:V),色谱柱为C18柱,柱温为40℃流速为1.0 mL/min,检测波长为240 nm。采用外标法进行定量。结果磺胺嘧啶钠的线性范围为12.5~200μg/mL,线性方程为Y=44.873X+11.999,r^2=1.000;甲氧苄啶的线性范围为2.5~40μg/mL,线性方程为Y=62.288X+4.0826,r^2=1.000,方法的线性关系良好,添加回收率均大于98%,RSD均小于2%。结论该方法简单、精确、可操作,可以用于测量复方磺胺嘧啶钠注射液中磺胺嘧啶钠与甲氧苄啶的含量。
An HPLC method was developed to determine the contents of sulfadiazine sodium and trimethoprim in Compound Sulfadiazine Sodium Injection.Using C18 column as stationary phase of 0.I%phosphoric solution-acetonitile(85:15),with flow rate of 1.0 mL/min,column oven temperature of 40 C and wavelength of 240 nm.It was quantified by external standard.The linear range of sulfadiazine sodium was between 12.5~200μg/mL and the regression equation was Y=44.873X+11.999,r^2=1.000.The linear range of trimethoprim was between 2.5~40μg/mL and the regression equation was Y=62.288X+4.0826,r^2=1.000.The result show that the linear relationship of the method was good,the recovery rates were more than 98%,and the RSD was less than 2%.This method was simple and accurate,and easy to operate,could be used for determining the contents of sulfadiazine sodum and trimethoprim in the Compound Sulfadiazine Sodium Injection.
作者
王庆红
孙真峥
廖欢
谢丽丽
陈锡龙
赵贵
WANG Qing-Hong;SUN Zhen-Zheng;LIAO Huan;XIE Li-Li;CHEN Xi-Long;ZHAO Gui(Guichou Prorincial Supervisory Instiute of Veterinary Drug and Feeds,Guiyang 550003,China)
出处
《中国兽药杂志》
2020年第6期61-65,共5页
Chinese Journal of Veterinary Drug
基金
家禽产品质量安全监测与控制关键技术集成研究(黔科合支撑[2019]2318号)
贵州牛奶质量安全主要风险因子控制关键技术研究(黔科合支撑[2019]2320号)。
