摘要
为提高低碳氮比污水中氮、磷的去除率,通过铁碳微电解耦合固相反硝化系统强化生物脱氮除磷的效果,分别考察了HRT、DO、pH对耦合系统中氮、磷去除效果的影响,并对铁碳颗粒(FC)、固体碳源颗粒(CC)和悬浮污泥(SS)的微生物群落结构进行了分析。结果表明:当进水C/N=1.5时,耦合系统的最佳运行参数为HRT=4 h、DO=2.0 mg·L-1、pH=7.0;此时NH4+-N、NO3--N、TN、TP的去除率分别为95.63%、93.48%、94.72%、99.10%。高通量测序分析结果表明:在门水平上,3个样本(FC、CC、SS)中的优势菌门为Proteobacteria、Actinobacteria和Bacteroidetes,其中具有反硝化脱氮功能的Proteobacteria在FC、CC、SS中分别占样本总数的72.66%、67.43%、68.66%;在纲水平上,SS中Alphaproteobacteria的相对丰度显著高于FC和CC,FC中的Gammaproteobacteria的相对丰度显著高于CC和SS,CC中的Gemmatimonadetes相对丰度明显高于FC和SS,生物除磷主要发生在CC中;在属水平上,Gemmobacter在FC、CC、SS中的相对丰度分别为25.50%、23.64%、32.53%,对异养反硝化过程起到重要作用。以上结果有助于提高对微电解-自养/异养反硝化除磷耦合系统中微生物生态学的理解。
In order to improve the removal efficiency of nitrogen and phosphorus in sewage with low carbonnitrogen ratio,the effects of HRT,DO and p H on nitrogen and phosphorus removal were investigated in a coupled system of iron-carbon microelectrolysis and solid denitrification.The microbial community structure in the iron carbon particles(FC),solid carbon source particles(CC) and suspended sludge(SS) were analyzed.The results showed that when the influent C/N ratio was 1.5,the optimal operating parameters of the coupling system were HRT=4 h,DO=2.0 mg·L-1,p H=7.0,and the removal rates of NH4+ NO3- -N,-N,TN,TP were 95.63%,93.48%,94.72% and 99.10%,respectively.High-throughput sequencing analysis demonstrated that at the phylum level,the dominant bacteria in the three samples(FC,CC,SS) were Proteobacteria,Actinobacteria and Bacteroidetes,respectively.Proteobacteria with denitrifying and denitrifying functions in FC,CC,SS were accounted for 72.66%,67.43% and 68.66% of the total sample,respectively.At the class level,the relative abundance of Alphaproteobacteria in SS was significantly higher than that in FC and CC,while Gammaproteobacteria in FC was significantly higher than that in CC and SS.Especially,the relative abundance of Gemmatimonadetes in CC was significantly higher than that of FC and SS,indicating that biological of phosphorus removal mainly occurred in CC.At the genus level,the relative abundances of Gemmobacter in FC,CC and SS were 25.50%,23.64% and 32.53%,respectively,which played an important role in denitrification.This study would be helpful to improve the understanding of microbial ecology in the coupled system of microelectrolysis-autotrophic/autotrophic denitrifying and phosphorus removal.
作者
唐婧
杨羽菲
陈金楠
TANG Jing;YANG Yufei;CHEN Jinnan(School of Municipal and Environmental Engineering,Shenyang Jianzhu University,Shenyang 110168,China;Department of Municipal and Environmental Engineering,Shenyang Urban Construction University,Shenyang 110167,China)
出处
《环境工程学报》
CAS
CSCD
北大核心
2020年第5期1224-1233,共10页
Chinese Journal of Environmental Engineering
基金
辽宁省教育厅重点攻关项目(lnzd201902)
辽宁省自然科学基金资助项目(20180551254)。
关键词
铁碳微电解
固相反硝化
脱氮除磷
低碳氮比
微生物群落
iron carbon microelectrolysis
solid denitrification
nitrogen and phosphorus removal
low carbon to nitrogen ratio
microbial community
