摘要
目的:研究葛根化学成分。方法:通过大孔树脂、MCI柱以及制备型HPLC进行分离纯化,根据理化性质和波谱数据进行结构鉴定。结果:分离纯化得到23个化合物,分别为美佛辛-4'-O-葡萄糖苷(1)、3'-羟基葛根素芹菜糖苷(2)、大豆苷元-4'-葡萄糖苷(3)、葛根素芹菜糖苷(4)、大豆苷元-4',7-二葡萄糖苷(5)、葛根素-6''-O-木糖苷(6)、3'-羟基葛根素(7)、3'-甲氧基葛根素(8)、tuberosin(9)、毛蕊异黄酮苷(10)、葛根苷B(11)、葛根苷A(12)、3'-甲氧基大豆苷(13)、染料木素-7-O-β-D-呋喃芹糖基-(1→6)-O-β-D-吡喃葡萄糖苷(14)、染料木苷(15)、刺芒柄花素(16)、formononetin-8-C-β-D-apiofuranosyl-(1→6)-O-β-Dglucopyranoside(17)、(+)-puerol B-2"-O-glucoside(18)、印度黄檀苷(19)、3'-甲氧基大豆苷元(20)、puerol B(21)、染料木素(22)、hydroxytuberosone(23)。结论:其中化合物10为首次从葛根中分离得到,研究结果丰富了葛根的化学成分。
Objective:To study the chemical constituents in the stems of Puerarialobata.Methods:The compounds were isolated by column chromatography of macroporous resin and MCI,and purified by MPLC.The structures were elucidated by means of physicochemical properties and spectral analysis.Results:Totally 23 compounds were separated and identified as mirificin-4'-O-glucoside(1),3'-hydroxymirificin(2),daidzein-4'-glucoside(3),mirificin(4),daidzein-4',7-diglucoside(5),puerarin 6''-O-xyloside(6),3'-hydroxy puerarin(7),3'-methoxypuerarin(8),tuberosin(9),calycosin-7-O-β-D-glucoside(10),pueroside B(11),puerosideA(12),3'-methoxydaidzin(13),ambocin(14),genistin(15),formononetin(16),formononetin-8-O-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside(17),(+)-puerol B-2"-O-glucoside(18),sissotrin(19),methoxydaidzein(20),puerol B(21),genistein(22)and hydroxytuberosone(23).Conclusion:Compound 10 is obtained for the first time from Puerarialobata.
作者
季鹏
张蕾
李民
Ji Peng;Zhang Lei;Li Min(Department of Pharmacy,Anhui Provincial Hospital,the First Affiliated Hospital of University of Science and Technology of China,Hefei 230001,China)
出处
《中国药师》
CAS
2020年第6期1184-1188,共5页
China Pharmacist
基金
国家自然科学基金青年科学基金项目(编号:81603339)。
关键词
葛根
化学成分
异黄酮
结构鉴定
Puerarialobata
Chemical constituents
Isoflavone
Structural identification
