摘要
【目的】探究腹泻犬源大肠埃希菌系统进化分群以及17种毒力因子的分布情况。【方法】采用PCR对成都地区分离的30株腹泻犬源大肠埃希菌进行菌株系统进化分群鉴定,同时对17种毒力基因进行检测。【结果】系统进化分群结果显示,30株分离株中A群系占53.33%(16/30),B1群系占23.33%(7/30),D群系占23.33%(7/30),未检出B2群系。17种毒力基因中fimC检出率最高(96.67%,29/30);其次为sitA(66.67%,20/30)、ompT(46.67%,14/30)、iss(16.67%,5/30)、fyuA(16.67%,5/30)、Irp2(16.67%,5/30)、iroN(13.33%,4/30);未检出毒力基因papA、tsh、hlyF、hlyA、cvaC、astA、estA、estB、eaeA和elt。【结论】成都地区分离的30株腹泻犬源大肠埃希菌系统进化分群多样,毒力基因检出率高且复杂。
[Objective]To investigate the distribution of phylogenetic clustering and virulence gene in Escherichia coli from diarrhea dogs in Chengdu.[Method]PCR method was used to identify the phylogenetic clustering of 30 strains isolated from diarrhea dogs from Chendu and the distribution of 17 virulence genes.[Result]Phylogenetic clustering results showed that the 30 strains isolated from diarrhea dogs,belong to group A(53.33%,16/30),group B1(23.33%7/30)and group D(23.33%7/30).Group B2 was not detected.Among the 17 virulence genes,fimC had the highest detection rate(96.67%,29/30),followed by sitA(66.67%,20/30),ompT(46.67%,14/30),iss(16.67%,5/30),fyuA(16.67%,5/30),Irp2(16.67%,5/30),iroN(13.33%,4/30).papA,tsh,hlyF,hlyA,cvaC,astA,estA,estB,eaeA and elt were not detected.[Conclusion]The 30 strains isolated from diarrhea dogs from Chengdu showed high diversity in phylogenetic clustering and high detection rate of virulence genes.
作者
朱子琦
魏斌
但佳明
龙佳丽
蒋绍齐
黄晓山
刘海峰
周紫峣
马晓平
彭广能
王玉飞
黄克和
钟志军
ZHU Zi-qi;WEI Bin;DAN Jia-ming;LONG Jia-li;JIANG Shao-qi;HUANG Xiao-shan;LIU Hai-feng;ZHOU Zi-yao;MA Xiao-ping;PENG Guang-neng;WANG Yu-fei;HUANG Ke-he;ZHONG Zhi-jun(Key Laboratory of Animal Disease and Human Health of Sichuan Province,Sichuan Agricultural University,College of Veterinary Medicine,Chengdu 611130,China;Animal Disease Prevention and Control Center of Qingxu County,Shanxi Province 030440,China;The Third Medical Center,General Hospital of the Chinese People’s Liberation Army,Beijing 100039,China;College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China)
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2020年第3期506-512,共7页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家重点研发计划项目(2018YFD0500900,2016YFD0501009)
成都大熊猫繁育研究基金会项目(CPF2017-05,CPF2015-4)
四川农业大学本科生科研兴趣培养项目(2019217,2019218)。
关键词
大肠埃希菌
系统进化分群
毒力基因
犬源
Escherichia coli
phylogenetic clustering
virulence gene
canine origin
