摘要
目的建立同时测定鸡蛋中7种色素含量的超高效液相色谱串联质谱(ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)法。方法鸡蛋样品用乙腈超声提取。经Phenomenex Kinetex F5(100 mm×3.0 mm,2.6μm)色谱柱分离,流动相A:0.1%的甲酸/水溶液;流动相B:0.1%的甲酸/乙腈溶液作为流动相梯度洗脱。电喷雾离子源(electrospray ionization,ESI)正离子多反应监测模式定量分析。结果7种色素在浓度1~20μg/L范围内线性关系良好,相关系数r 2≥0.998。叶黄素、角黄素、苏丹红Ⅰ、苏丹红Ⅱ、苏丹红Ⅲ、苏丹红Ⅳ的检出限均为0.1μg/kg,核黄素的检出限为0.2μg/kg。7种色素在加标量2~10μg/kg范围内,回收率为80.2%~106.7%,相对标准偏差为1.5%~6.0%。结论该方法准确、快速、高效,可用于鸡蛋中色素的定性、定量分析。
Objective To establish the method for the simultaneous determination of 7 pigments in eggs by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Methods Egg samples were extracted by acetonitrile ultrasound,and separated by Phenomenex Kinetex F5(100 mm×3.0 mm,2.6 m)column.Mobile phase A:0.1% formic acid/aqueous solution and mobile phase B:0.1% formic acid/acetonitrile solution were used as mobile phase for gradient elution.Positive ion multi-reaction monitoring mode of electrospray ionization(ESI)was used for quantitative analysis.Results The linearity of the 7 pigments was good in the range of 1-20 g/L,and the correlation coefficient r 2≥0.998.The detection limits of lutein,canthaxanthin,Sudan Ⅰ,Sudan Ⅱ,Sudan Ⅲ and Sudan Ⅳ were 0.1 g/kg,and that of riboflavin was 0.2 g/kg.The recoveries of 7 pigments were 80.2%-106.7% and the relative standard deviations were 1.5%-6.0% in the range of 2-10 g/kg.Conclusion The proposed method is fast,accurate and efficiency,which is suitable for qualitative and quantitative analysis of endogenous and exogenous pigments in eggs.
作者
冯永巍
杨总
孙嵇成
沈晓芳
汪振炯
FENG Yong-Wei;YANG Zong;SUN Ji-Cheng;SHEN Xiao-Fang;WANG Zhen-Jiong(Wuxi Institute for Food Control,Wuxi 214142,China;Shanghai AB Sciex Analytical Instrument Trading Co.Ltd,Shanghai 200335,China;School of Food Science and Technology,Jiangnan University,Wuxi 214122,China;School of Food Science,Nanjing Xiaozhuang University,Nanjing 211171,China)
出处
《食品安全质量检测学报》
CAS
2020年第11期3495-3499,共5页
Journal of Food Safety and Quality
关键词
色素
超高效液相色谱-串联质谱
鸡蛋
pigment
ultra performance liquid chromatography-tandem mass spectrometry
egg
